| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,042,202 | 0 | A | C | 53.8% | 7.1 / 14.8 | 13 | T1262P (ACC→CCC) | yeeJ | inverse autotransporter adhesin YeeJ |
| Reads supporting (aligned to +/- strand): ref base A (6/0); new base C (0/7); total (6/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.83e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGTGCATTTTATTGGCGACACAGCGGCGGCAAAAATTATCGAGTTGGCGCCTGTCCCAGACAGCATAATCGCCGGTACCCCGCAGAACAGCTCCGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGAACTT > NZ_CP009273/2042126‑2042287 | tgtgCATTTTATTGGCGACACAGCGGCGGCAAAAATTATCGAGTTGGCGCCTGTCCCAGACAGCATAATCGCCGGTACCCCGCAGAACAg > 1:137255/1‑90 (MQ=255) gACACAGCGGCGGAAAAAATTATCGGGTGGGCCCCTGCCCCAGACAGCATAACCGCCGGTCCCCCGCAGAACAGCTCCGGCAGCGtcatc < 1:814889/90‑1 (MQ=255) ggcggaAAAATTTATGGGGTGGGCCCCTGCCCCAGACACAAAAACCGCCGGTCCCCCGCAAAACAGCTCCGGCAGCGCCATCACCGCcac < 1:544546/90‑1 (MQ=255) aaaaaTTATCGAGTTGGCGCCTGTCCCAGACAGCATAATCGCCGGTACCCCGCAGAACAGCTCCGGCAGCGTCATCACCGCCACAGTCGt > 2:437464/1‑90 (MQ=255) aaTTATCGAGTGGGCGCTTCCCCCAGCCAGAATAACCGCCGGTCCCCCGCAGAACAGCTCCGGCGGCGTCACCACCGCCACAGTCGTTGa < 1:187228/90‑1 (MQ=255) ccccAAACAGCATAACCGCCGGTCCCCCGAAGAACAGCTCCGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGa < 2:1497724/89‑1 (MQ=255) ccAGACAGCATAATCGCCGGTCCCCCGCAGAACAGCTCGGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGaaa < 2:137255/90‑1 (MQ=255) aGCATAATCGCCGGTCCCCCGAAAAACACCTCCGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGtgtg < 1:109829/90‑1 (MQ=255) gCATAATCGCCGGTACCCCGCAGAACAGCTCCGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGa > 1:1497724/1‑90 (MQ=255) gCATAATCGCCGGTACCCCGCAGAACAGCTCCGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGa > 2:1014998/1‑90 (MQ=255) gCATAATCGCCGGTACCCCGCAGAACAGCTCCGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGa > 2:1337754/1‑90 (MQ=255) aTAACCGCGGGTCCCCCGCGAAACAGCTCGGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACt < 2:943780/90‑1 (MQ=255) cGGTACCCCGCAGAACAGCTCCGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGAACtt > 2:979468/1‑90 (MQ=255) | TGTGCATTTTATTGGCGACACAGCGGCGGCAAAAATTATCGAGTTGGCGCCTGTCCCAGACAGCATAATCGCCGGTACCCCGCAGAACAGCTCCGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGAACTT > NZ_CP009273/2042126‑2042287 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |