| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,149,203 | 0 | A | C | 53.3% | 13.0 / 18.4 | 15 | T154P (ACC→CCC) | mdtB | multidrug efflux RND transporter permease subunit MdtB |
| Reads supporting (aligned to +/- strand): ref base A (7/0); new base C (0/8); total (7/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.55e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.72e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AACTTGTTGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAGTGAA‑CCCGGCAGATCCGCCGATCATGACGCTCGCCGTCACCTCAACCGCCATGCCGATGACGCAAGTGGAAGATATGGTGGAAACCCGCGTCGCGCAGAAAATCTCGCAGATTTCCGGCG > NZ_CP009273/2149119‑2149284 | aaCTTGTTGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAGTGAA‑CCCGGCAGATCCGCCGATCATGACGCTCGCCGTCACCTCa > 1:298252/1‑90 (MQ=255) ccccGCCGTTTAACAAAAAAGTGAC‑CCCGGAAGACCCGCCGATCAGGCCGCCCCCCGCCCCCTCAACCGCCATGCCGATGACGCAAGTgg < 2:656339/89‑1 (MQ=255) ccgccgTTTTACAGAAAAGTGAC‑CCGGGCAGACCCGCCGATCATGACGCTCGCCGTCCCCTCAACCGCCATGCCGATGACGCAAGTGGaa < 2:168058/90‑1 (MQ=255) ccgGTTTACAGCAAAGTGAA‑CCCGGCAGATCCGCCGATCATGACGCTCGCCGTCACCTCAACCGCCATGCCGATGACGCAAGTGGAAGat > 1:923285/1‑90 (MQ=255) gttttCCAGAAAAGTGAC‑CCGGGCGGACCCGCCTATTAGGGCCCTCGCCGGCCCCACAACCGCCATGCCGATGACGCAAGTGGAAGatat < 2:923285/88‑1 (MQ=255) gttttACAGAAAAGTGAC‑CCCGGAAGACCCGCCAATCATGCCGCTCGCCGCCCCCTCAACCGCCATGCCGATGACGCAAGTGGAAGatat < 2:28551/88‑1 (MQ=255) gTTTAAAACAAAGGTAACCCCGGAAGACCCGCCGATCATGCCGCTCGCCGTCCCCTCAACCGCCATGCCGATGACGCAAGTGGAAGatat < 2:1223798/90‑1 (MQ=255) aaGTGAA‑CCCGGCAGATCCGCCGATCATGACGCTCGCCGTCACCTCAACCGCCATGCCGATGACGCAAGTGGAAGATATGGTGGAAAccc > 1:1539613/1‑90 (MQ=255) aa‑CCCGGCAGATCCGCCGATCATGACGCTCGCCGTCACCTCAACCGCCATGCCGATGACGCAAGTGGAAGATATGGTGGAAACCCGCGTc > 1:995109/1‑90 (MQ=255) gccgATCATGACGCTCGCCGTCCCCTCAACCGCCATGCCGATGACGCAAGTGGAAGATATGGTGGAAACCCGCGTCGCGCAGAAAAtctc < 2:228870/90‑1 (MQ=255) gtCAGGACGCTCGCCGTCCCCTCACCCGCCATGCCGATGACGCAAGTGGAAGATATGGTGGAAACCCGCGTCGCGCAGAAAATCTCGCAg < 2:814629/89‑1 (MQ=255) ccGCTCCCCGCCCCCTCAACCGCCATGCCGATGACGCAAGTGGAAGATATGGTGGAAACCCGCGTCGCGCAGAAAATCTCGCAGATTTcc < 2:1511216/89‑1 (MQ=255) cTCGCCGTCACCTCAACCGCCATGCCGATGACGCAAGTGGAAGATATGGTGGAAACCCGCGTCGCGCAGAAAATCTCGCAGATTTCCGGc > 1:158045/1‑90 (MQ=255) cTCGCCGTCACCTCAACCGCCATGCCGATGACGCAAGTGGAAGATATGGTGGAAACCCGCGCCGCGCAGAAAATCTCGCAGATTTCCGGc > 1:883823/1‑90 (MQ=255) tCGCCGTCACCTCAACCGCCATGCCGATGACGCAAGTGGAAGATATGGTGGAAACCCGCGTCGCGCAGAAAATCTCGCAGATTTCCGGCg > 2:952339/1‑90 (MQ=255) | AACTTGTTGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAGTGAA‑CCCGGCAGATCCGCCGATCATGACGCTCGCCGTCACCTCAACCGCCATGCCGATGACGCAAGTGGAAGATATGGTGGAAACCCGCGTCGCGCAGAAAATCTCGCAGATTTCCGGCG > NZ_CP009273/2149119‑2149284 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |