Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	49,912	G→T	W30C (TGG→TGT)	folA →	type 3 dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	49,912	0	G	T	100.0%	232.6 / NA	66	W30C (TGG→TGT)	folA	type 3 dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (28/38);  total (28/38)

GATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAA  >  NZ_CP009273/49825‑49997                                                                                        |                                                                                      gATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGttt                                                                                     <  2:1009563/90‑1 (MQ=255)   tCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTa                                                                                   <  2:677217/90‑1 (MQ=255)   tCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTa                                                                                   >  1:1544191/1‑90 (MQ=255)    cAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGGAAACGCCATGCCGGGGAACCTGCCTGCCGATCTCGCCTGTTTTaa                                                                                  >  2:945041/1‑90 (MQ=255)    cAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTaa                                                                                  >  2:927017/1‑90 (MQ=255)    cAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTaa                                                                                  >  2:405242/1‑90 (MQ=255)     aGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTaaa                                                                                 >  1:687605/1‑90 (MQ=255)          gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCaa                                                                            <  2:883481/90‑1 (MQ=255)          gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCaa                                                                            <  1:834428/90‑1 (MQ=255)          gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCaa                                                                            <  1:998582/90‑1 (MQ=255)          gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCaa                                                                            <  2:131388/90‑1 (MQ=255)          gATTCCGGCGTTAGCGGTAGATCGCGTTACCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCaa                                                                            <  2:470182/90‑1 (MQ=255)           aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAac                                                                           <  2:467231/90‑1 (MQ=255)           aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAac                                                                           >  1:204672/1‑90 (MQ=255)              gcggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACAcc                                                                        <  1:294658/90‑1 (MQ=255)              gcggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACAcc                                                                        <  1:1392484/90‑1 (MQ=255)               cggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCt                                                                       <  1:405242/90‑1 (MQ=255)                ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCaa                                                                            >  2:1529447/1‑84 (MQ=255)                ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCtt                                                                      >  2:582989/1‑90 (MQ=255)                ggcgTTAGCGGTAGATCGCGTTATCGGAATGGAAAACGCCATGCCGTGAAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCaa                                                                            <  1:1529447/84‑1 (MQ=255)                 gcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTta                                                                     <  2:204672/90‑1 (MQ=255)                    ttAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCaa                                                                            >  1:27809/1‑80 (MQ=255)                    ttAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCaa                                                                            <  2:27809/80‑1 (MQ=255)                     tAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTtaaata                                                                 >  2:897137/1‑90 (MQ=255)                     tAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTtaaata                                                                 <  2:565733/90‑1 (MQ=255)                        cGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAAc                                                              <  2:1219344/90‑1 (MQ=255)                          gTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTCCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAAccc                                                            <  1:51592/90‑1 (MQ=255)                           tAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCg                                                           >  2:1105312/1‑90 (MQ=255)                            aGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGt                                                          <  1:1384141/90‑1 (MQ=255)                                 gcgTTATCGGCATGGAAAACGCCCTGCCGTGGAACCTGCCTGCCGGTCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTa                                                     >  1:1290064/1‑90 (MQ=255)                                 gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAAccc                                                            >  1:1530058/1‑83 (MQ=255)                                 gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAAccc                                                            <  2:1530058/83‑1 (MQ=255)                                 gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTa                                                     >  2:148054/1‑90 (MQ=255)                                 gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTa                                                     >  2:872046/1‑90 (MQ=255)                                 gcgTTATCGGCATGGAAAACCCCCTGCCGTGGAACCTGCCTGCCCATCTCGCCTGTTTTAAACGCAAAACCTTAAATAAACCCGTggata                                                     >  1:1384111/1‑86 (MQ=255)                                    ttATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATgg                                                  >  2:27676/1‑90 (MQ=255)                                    ttATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATgg                                                  >  1:166165/1‑90 (MQ=255)                                      ttCGGCATGAAAACCGCCATGCCGGGGACCCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgc                                                <  1:1584718/89‑1 (MQ=255)                                       ccGGCATGGAAAACGCCATGCGGGGGACCCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACCCCTTAAATAAACCCGTGATTATGGgcc                                               <  1:945041/89‑1 (MQ=255)                                        cGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccg                                              <  2:253203/90‑1 (MQ=255)                                           cATGGAAACCCCAATGCGGTGAAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCa                                           <  1:340899/90‑1 (MQ=255)                                            aTGAAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAt                                          <  2:1290064/90‑1 (MQ=255)                                            aTGAAAAACGCCATCCCGTGAACCCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAt                                          <  1:1396468/90‑1 (MQ=255)                                             tGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAAcc                                                             <  2:96636/70‑1 (MQ=255)                                             tGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAAcc                                                             >  1:96636/1‑70 (MQ=255)                                                    cGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccgc                                             >  1:1604138/1‑79 (MQ=255)                                                    cGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccgc                                             <  2:1604138/79‑1 (MQ=255)                                                        aTGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCaa                                                                            <  1:1170429/44‑1 (MQ=255)                                                        aTGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCaa                                                                            >  2:1170429/1‑44 (MQ=255)                                                         tGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatcaa                             >  2:1197674/1‑90 (MQ=255)                                                         tGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatcaa                             >  2:537848/1‑90 (MQ=255)                                                           ccGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatcaatc                           <  2:1107207/90‑1 (MQ=255)                                                             gTGGACCCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCgg                         <  2:1136858/90‑1 (MQ=255)                                                             gTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCgg                         >  2:1047047/1‑90 (MQ=255)                                                                   cctccctgCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTcc                   <  1:875994/90‑1 (MQ=255)                                                                    ctccctgccGATCTCGCCTGTTTTAAACGCAACCCCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCg                  <  1:224618/87‑1 (MQ=255)                                                                     tgcctgccGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGt                 >  2:1309239/1‑90 (MQ=255)                                                                     tgcctgccGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGt                 >  1:1105359/1‑90 (MQ=255)                                                                      gcctgccGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTcc                   <  2:1592746/87‑1 (MQ=255)                                                                      gcctgccGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTcc                   >  1:1592746/1‑87 (MQ=255)                                                                         tgccGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGcc             <  2:1023452/90‑1 (MQ=255)                                                                              aTCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGAc        <  2:637565/90‑1 (MQ=255)                                                                              aTCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGAc        <  1:1105312/90‑1 (MQ=255)                                                                                   gCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCaaa   <  2:1105359/90‑1 (MQ=255)                                                                                   gCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCaaa   <  2:401571/90‑1 (MQ=255)                                                                                   gCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCaaa   <  1:31430/90‑1 (MQ=255)                                                                                    ccTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCaaaa  >  1:886073/1‑90 (MQ=255)                                                                                        |                                                                                      GATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAA  >  NZ_CP009273/49825‑49997

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑