| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 360,759 | 0 | T | G | 53.8% | 2.2 / 15.5 | 13 | T329P (ACC→CCC) | lacI | DNA‑binding transcriptional repressor LacI |
| Reads supporting (aligned to +/- strand): ref base T (0/6); new base G (7/0); total (7/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.83e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.68e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCACCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTG > NZ_CP009273/360692‑360847 | cGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGGACCCGGGGGGGTTTT‑TTTTTACCCGTgaga > 2:55415/1‑90 (MQ=255)cGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGGATTGGGGACCAGGGGGGGTTTTTTTTTTACCAGGgag > 2:493819/1‑90 (MQ=255) ccAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCACCAGGGTGGTTTTTCTTTTCACCAGTGAGACgg < 1:401115/90‑1 (MQ=255) gCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCACCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCa < 2:351184/90‑1 (MQ=255) gCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCACCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACa < 1:310391/90‑1 (MQ=255) gAATCGGCCAACGCGCGGGGAGAGGCGGTTTTCGTATTTGGCACCAGGGGGGGTTTTTTTTTTACCCGGGAGACGGGGAACAGCTGATTg > 2:92186/1‑90 (MQ=255) aTCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGGACCAGGGGGGGTTTTTTTTTTACCAGTGGGACGGGGAACAGCTGATTGcc > 2:116853/1‑90 (MQ=255) aTCGGCCAACGCGCGGGGAGAGGCGGTTTGCGGATTGGGGGCCAGGGGGGGTTTTTTTTTTTCCAGTGAGACGGGGAACAGCTGATTGcc > 2:265103/1‑90 (MQ=255) aTCGGCCAACGCGCGGGGAGAGGCGGTTTGCGGATTGGGCACCAGGGGGGGTTTTTTTTTTACCAGTGAGACGGGCAAAAGCTGATTGcc > 2:166799/1‑90 (MQ=255) cGGCCAACGCGCGGGGAGAGGCGGTTTTCGGATTTGGGACCCGGGGGGGTTTTTTTTTTACCCGGGGGGGGGGGAACAGCTGGTTGcccc > 2:261940/1‑89 (MQ=255) gcgGGGAGAGGCGGTTTGCGTATTGGGCACCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTg < 2:330241/90‑1 (MQ=255) agGCGGTTTGCGTATTGGGCACCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTgag < 2:250804/90‑1 (MQ=255) tGGGCACCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGc < 2:596189/90‑1 (MQ=255) ggtggtTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCt < 1:175788/90‑1 (MQ=255) gtggtTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTg > 2:251146/1‑90 (MQ=255) | CGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCACCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTG > NZ_CP009273/360692‑360847 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |