Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,548,2570TG53.3% 13.0 / 15.3 15Y557S (TAC→TCC) maeAmalate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (3/4);  new base G (8/0);  total (11/4)
Fisher's exact test for biased strand distribution p-value = 2.56e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.15e-03
Rejected as consensus: Frequency below/above cutoff threshold.

GATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCT  >  NZ_CP009273/1548169‑1548332
                                                                                        |                                                                           
gATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTa                                                                            >  1:347782/1‑90 (MQ=255)
     gaTCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCgg                                                                       >  1:52317/1‑90 (MQ=255)
                    gagGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGGATTCGGCTTGCCAGAAATTAt                                                        >  1:470526/1‑90 (MQ=255)
                          aaTAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGGATTCGGGTTGCCAGAAATTATCggcca                                                  >  1:522308/1‑86 (MQ=255)
                           aTAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAAt                                                 <  2:52317/90‑1 (MQ=255)
                                                  gggCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCag                          <  2:347782/90‑1 (MQ=255)
                                                  gggCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAAATATCGTCAATGGCCCGTTGCAGGGCTTCGGccg                          >  2:104376/1‑88 (MQ=255)
                                                  gggCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGGATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCag                          >  1:463603/1‑90 (MQ=255)
                                                    gcgcAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGGATTCGGCTTGCCCGAAAATATCCTCAATGGCCTGTTGCAGGGGTTCGGGagag                        >  2:198338/1‑90 (MQ=255)
                                                    gcgcAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGGATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCagag                        >  1:194683/1‑90 (MQ=255)
                                                           cTTAGAGGGAGGTACGGCGGTAGTCGCGGTTTTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCa                 <  2:194683/90‑1 (MQ=255)
                                                                   gAGGTACGGCGGTAGTCGCGGGATTCGGCTTGCCAGAAATTATCGTCAATGGCCCGTTGCAGGGCTTCGGCAGAGGGTTTCACCGCCACg         >  1:196382/1‑90 (MQ=255)
                                                                        aCGGCGGTAGTCGCGGGATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGGTTTTACCGCCACGCCTtg    >  1:406081/1‑90 (MQ=255)
                                                                         cggcggTAGTCGCGGGATTTGGCTTGCCAGAAAATATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGGTTTTACCCCCCCGCCTtgc   >  2:104937/1‑90 (MQ=255)
                                                                          ggcggTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGTTTTTCACCGCCACGCCTtgct  <  2:41339/90‑1 (MQ=255)
                                                                                        |                                                                           
GATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCT  >  NZ_CP009273/1548169‑1548332

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: