| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,734,269 | 0 | T | G | 60.9% | 6.5 / 24.2 | 23 | V402G (GTG→GGG) | avtA | valine‑‑pyruvate transaminase |
| Reads supporting (aligned to +/- strand): ref base T (0/9); new base G (14/0); total (14/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.22e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.74e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATT > NZ_CP009273/3734186‑3734358 | aGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGAt > 2:571999/1‑90 (MQ=255) gggCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGAtt > 2:130262/1‑90 (MQ=255) ggATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGATTCTGGc > 2:15150/1‑90 (MQ=255) tAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGa < 2:328419/90‑1 (MQ=255) aaCCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAag < 1:571999/90‑1 (MQ=255) tGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGGAGAATCTGGGGGgagaaaaa > 2:555522/1‑86 (MQ=255) tGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGATTCTGGCGGGAGAGATa > 2:428822/1‑90 (MQ=255) cGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCt < 1:420566/90‑1 (MQ=255) aaTGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGATTCTGGGGGGAGAGATAGAAAAAGCCTGGGCTg > 2:335698/1‑90 (MQ=255) tATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGATTTTGGCGGGAGAGATAGAAAAAGCCTGGGCTGAAAg > 2:387198/1‑90 (MQ=255) aTGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGt < 1:335698/90‑1 (MQ=255) tGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTc > 1:104295/1‑90 (MQ=255) gTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGATTCTTGCGGAAGAGATAGAAAAAGGCCGGGGGGGAAGGCACTAAAAAATGGCt > 2:278893/1‑90 (MQ=255) gagCCGGAGAAAATTGAGGCGGGGGGGAAGATTTTGGCGGGAGAGATAGAAAGAGCCTGGGGTGAAAGTCACTAAAAATTGCCTGATgcg > 2:405254/1‑90 (MQ=255) gagCCGGAGAAAATTGAGGCGGGGGGGAAGATTCTGGCGGAAGAGATAGAAAAAGCCCGGGGTGAAAGGCACTAAAAAATTCCTGATgcg > 2:220010/1‑90 (MQ=255) agCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATgcgc < 1:114000/90‑1 (MQ=255) agCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATgcgc < 1:555522/90‑1 (MQ=255) agCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATgcgc < 1:130651/90‑1 (MQ=255) gCCGGAGAAAATTGAGGCGGGGGGGAAGATTCTGGCGGGAGAGATAGAAAAAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCt > 2:79004/1‑90 (MQ=255) aaaTTGAGGCGGGGGGGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGGTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTAt > 1:450150/1‑90 (MQ=255) aTTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCa < 1:53380/90‑1 (MQ=255) gAGGCGGGGGGGAAGATTCTGGCGGAAGAGAAAGAAAGAGCCTGGGGTGAAAGTAACTAAAAATTGCCTGAATCGCGACGCTTATCAGGc > 2:120675/1‑90 (MQ=255) gggggTGAAGATTCTGGAGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACa < 1:586347/90‑1 (MQ=255) tGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGAtt < 2:84743/90‑1 (MQ=255) | AGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATT > NZ_CP009273/3734186‑3734358 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |