| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 49,883 | C→T | P21S (CCG→TCG) | folA → | type 3 dihydrofolate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 49,883 | 0 | C | T | 97.7% | 132.7 / NA | 44 | P21S (CCG→TCG) | folA | type 3 dihydrofolate reductase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (21/22); minor base G (1/0); total (22/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01 | |||||||||||
GACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAT > NZ_CP009273/49796‑49957 | gACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGTCg > 1:279632/1‑90 (MQ=255)gACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGTCg > 1:86642/1‑90 (MQ=255) ttttATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGTCGTGGAAcctg < 1:80845/90‑1 (MQ=255) tCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGTCGTGGAACctgcctgc > 2:293044/1‑90 (MQ=255) tCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGTCGTGGAACctgcctgc < 2:76046/90‑1 (MQ=255) tcAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGTCGTGGAACCTGCCTGCCGATCTCGcc < 2:377551/90‑1 (MQ=255) tcAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGTCGTGGAACCTGCCTGCCGATCTCGcc < 1:293044/90‑1 (MQ=255) tcAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGTCGTGGAACCTGCCTGCCGATCTCGcc < 2:439622/90‑1 (MQ=255) tcAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGTCGTGGAACCTGCCTGCCGATCTCGcc < 1:216474/90‑1 (MQ=255) tCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGTCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTa > 2:316042/1‑90 (MQ=255) tCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGTCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTa < 1:391069/90‑1 (MQ=255) cAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGTCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTaa > 1:59268/1‑90 (MQ=255) cAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGTCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTaa > 2:420730/1‑90 (MQ=255) cAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGTCGGGGAACCTGCCTGCCGATCTCGCCTGGTTTaa > 2:380134/1‑90 (MQ=255) aGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGTCGTGGAACCTGCCTGCCGATCTCGcc < 2:391368/81‑1 (MQ=255) aGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGTCGTGGAACCTGCCTGCCGATCTCGcc > 1:391368/1‑81 (MQ=255) aGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGTCGTGGAACCTGCCTGCCGATCTCGCCTg < 1:435178/83‑1 (MQ=255) aGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGTCGTGGAACCTGCCTGCCGATCTCGCCTg > 2:435178/1‑83 (MQ=255) aGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGTCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTa > 2:249072/1‑88 (MQ=255) aGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGTCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTa < 1:249072/88‑1 (MQ=255) gTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCAGGGAAAACGCCATGTCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAAc < 2:130070/90‑1 (MQ=255) gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGTCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCaa < 1:580563/90‑1 (MQ=255) gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGTCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCaa < 1:564449/90‑1 (MQ=255) gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGTCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCaa < 1:421179/90‑1 (MQ=255) gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGTCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCaa < 1:328971/90‑1 (MQ=255) aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGTCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAac > 1:283323/1‑90 (MQ=255) cggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGTCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCt < 1:250152/90‑1 (MQ=255) ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGTCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCtt > 1:446077/1‑90 (MQ=255) ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGTCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCtt > 2:378469/1‑90 (MQ=255) cgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGTCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTtaa > 1:25363/1‑90 (MQ=255) tAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGTCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTtaaata > 2:11882/1‑90 (MQ=255) tCGCGTTATCGGCATGGAAAACGCCATGTCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGAt > 2:534022/1‑90 (MQ=255) gcgTTATCGGCATGGAAAACGCCATGTCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCaa > 2:85657/1‑67 (MQ=255) gcgTTATCGGCATGGAAAACGCCATGTCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCaa < 1:85657/67‑1 (MQ=255) gcgTTATCGGCATGGAAAACGCCATGTCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTa > 2:229766/1‑90 (MQ=255) gcgTTATCGGCATGGAAAACGCCATGTCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTa > 1:505235/1‑90 (MQ=255) ttATCGGCATGGAAAACGCCATGTCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATgg < 1:69618/90‑1 (MQ=255) ttATCGGCATGGAAAACGCCATGTCGTGGAACCTGCCTGCCGATCTCGCCTGGGTTAAACGCAACACCTTAAATAAACCCGTGATTATgg > 2:386995/1‑90 (MQ=255) aTCGGCATGGAAAACGCCATGTCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAACCCCGTGATTATGGgc < 2:166244/90‑1 (MQ=255) aTCGGCATGGAAAACGCCATGTCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgc < 2:579779/90‑1 (MQ=255) aTGGAAAACGCCATGTCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTa > 2:39253/1‑49 (MQ=255) aTGGAAAACGCCATGTCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTa < 1:39253/49‑1 (MQ=255) aTGGAAAACGCCATGTCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAt < 1:332854/90‑1 (MQ=255) aTGGAAAACGCCATGGCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGGTTATGGgccgccct > 1:52664/1‑88 (MQ=255) | GACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAT > NZ_CP009273/49796‑49957 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |