Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	214,743	A→C	Y174D (TAC→GAC)	proS ←	proline‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	214,743	0	A	C	100.0%	92.4 / NA	25	Y174D (TAC→GAC)	proS	proline‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (13/12);  total (13/12)

GAACCGGTGTCGGCTTGTACGGCGCGGAAATCCAGCCCCATGCGGCTGAAGATTTTGCTGTAGGCCGCATACATTGCATCGTAGGTTTCCTGCAGGGATTCCTGAGAAGTATGGAAAGAGTAAGCATCTTTCATCAGGAATTCGCGGGAACGCATGACGCCGAAACG  >  NZ_CP009273/214661‑214827                                                                                   |                                                                                     gAACCGGTGTCGGCTTGTACGGCGCGGAAATCCAGCCCCATGCGGCTGAAGATTTTGCTGTAGGCCGCATACATTGCATCGTCGGTTTcc                                                                               >  1:288965/1‑90 (MQ=255)        tgtCGGCTTGTACGGCGCGGAAATCCAGCCCCATGCGGCTGAAGATTTTGCTGTAGGCCGCATACATTGCATCGTCGGTTTCCTGCAggg                                                                        <  1:538493/90‑1 (MQ=255)           cGGCTTGTACGGCGCGGAAATCCAGCCCCATGCGGCTGAAGATTTTGCTGTAGGCCGCATACATTGCATCGTCGGTTTCCTGCAGGGAtt                                                                     >  1:473438/1‑90 (MQ=255)                   aCGGCGCGGAAATCCAGCCCCATGCGGCTGAAGATTTTGCTGTAGGCCGCATACATTGCATCGTCGGTTTCCTGCAGGGATTCCTGAGaa                                                             <  2:176616/90‑1 (MQ=255)                      gcgcGGAAATCCAGCCCCATGCGGCTGAAGATTTTGCTGTAGGCCGCATACATTGCATCGTCGGTTTCCTGCAGGGATTCCTGAGAAGTa                                                          >  2:548288/1‑90 (MQ=255)                      gcgcGGAAATCCAGCCCCATGCGGCTGAAGATTTTGCTGTAGGCCGCATACATTGCATCGTCGGTTTCCTGCAGGGATTCCTGAGAAGTa                                                          >  1:467806/1‑90 (MQ=255)                       cgcgGAAATCCAGCCCCATGCGGCTGAAGATTTTGCTGTAGGCCGCATACATTGCATCGTCGGTTTCCTGCAGGGATTCCTGAGAAGTAt                                                         >  1:236391/1‑90 (MQ=255)                           gAAATCCAGCCCCATGCGGCTGAAGATTTTGCTGTAGGCCGCATACATTGCATCGTCGGTTTCCTGCAGGGATTCCTGAGAAGTATGGaa                                                     <  1:513999/90‑1 (MQ=255)                            aaaTCCAGCCCCATGCGGCTGAAGATTTTGCTGTAGGCCGCATACATTGCATCGTCGGTTTCCTGCAGGGATTCCTGAGAAGTATGGaaa                                                    <  1:4255/90‑1 (MQ=255)                            aaaTCCAGCCCCATGCGGCTGAAGATTTTGCTGTAGGCCGCATACATTGCATCGTCGGTTTCCTGCAGGGATTCCTGAGAAGTATGGaaa                                                    <  1:151538/90‑1 (MQ=255)                                  aGCCCCATGCGGCTGAAGATTTTGCTGTAGGCCGCATACATTGCATCGTCGGTTTCCTGCAGGGATTCCTGAGAAGTATGGAAAGAGTaa                                              >  1:16735/1‑90 (MQ=255)                                   gCCCCATGCGGCTGAAGATTTTGCTGTAGGCCGCATACATTGCATCGTCGGTTTCCTGCAGGGATTCCTGAGAAGTATGGAAAGAGTa                                               <  1:145258/88‑1 (MQ=255)                                   gCCCCATGCGGCTGAAGATTTTGCTGTAGGCCGCATACATTGCATCGTCGGTTTCCTGCAGGGATTCCTGAGAAGTATGGAAAGAGTa                                               >  2:145258/1‑88 (MQ=255)                                       cATGCGGCTGAAGATTTTGCTGTAGGCCGCATACATTGCATCGTCGGTTTCCTGCAGGGATTCCTGAGAAGTATGGAAAGAGTAAGCATc                                         <  1:74557/90‑1 (MQ=255)                                        aTGCGGCTGAAGATTTTGCTGTAGGCCGCATACATTGCATCGTCGGTTTCCTGCAGGGAt                                                                      <  1:237662/60‑1 (MQ=255)                                        aTGCGGCTGAAGATTTTGCTGTAGGCCGCATACATTGCATCGTCGGTTTCCTGCAGGGAt                                                                      >  2:237662/1‑60 (MQ=255)                                        aTGCGGCTGAAGATTTTGCTGTAGGCCGCATACATTGCATCGTCGGTTTCCTGCAGGGATTCCTGAGAAGTATGGAAAGAGTAAGCATCt                                        >  1:144033/1‑90 (MQ=255)                                        aTGCGGCTGAAGATTTTGCTGTAGGCCGCATACATTGCATCGTCGGTTTCCTGCAGGGATTCCTGAGAAGTATGGAAAGAGTAAGCATCt                                        >  1:212148/1‑90 (MQ=255)                                        aTGCGGCTGAAGATTTTGCTGTAGGCCGCATACATTGCATCGTCGGTTTCCTGCAGGGATTCCTGAGAAGTATGGAAAGAGTAAGCATCt                                        >  2:178604/1‑90 (MQ=255)                                               tGAAGATTTTGCTGTAGGCCGCATACATTGCATCGTCGGTTTCCTGCAGGGATTCCTGAGAAGTATGGAAAGAGTAAGCATCTTtcatca                                 <  1:79954/90‑1 (MQ=255)                                                 aaGATTTTGCTGTAGGCCGCATACATTGCATCGTCGGTTTCCTGCAGGGATTCCTGAGAAGTATGGAAAGAGTAAGCATCTTTCATCAgg                               <  1:327876/90‑1 (MQ=255)                                                    aTTTTGCTGTAGGCCGCATACATTGCATCGTCGGTTTCCTGCAGGGATTCCTGAGAAGTATGGAAAGAGTAAGCATCTTTCATCAGGAAt                            <  2:16735/90‑1 (MQ=255)                                                            gTAGGCCGCATACATTGCATCGTCGGTTTCCTGCAGGGATTCCTGAGAAGTATGGAAAGAGTAAGCATCTTTCATCAGGAATTCGCGGGa                    >  1:514796/1‑90 (MQ=255)                                                                             cATCGTCGGTTTCCTGCAGGGATTCCTGAGAAGTATGGAAAGAGTAAGCATCTTTCATCAGGAATTCGCGGGAACGCATGACGCCGAAAc   <  2:144033/90‑1 (MQ=255)                                                                              aTCGTCGGTTTCCTCCAGGGATTCCTGAGAAGTATGGAAAGAGTAATCATCTTTCATCAGGAATTCGCGGGGACGCATGAAGCCGAAAcg  >  2:292544/1‑90 (MQ=255)                                                                                   |                                                                                     GAACCGGTGTCGGCTTGTACGGCGCGGAAATCCAGCCCCATGCGGCTGAAGATTTTGCTGTAGGCCGCATACATTGCATCGTAGGTTTCCTGCAGGGATTCCTGAGAAGTATGGAAAGAGTAAGCATCTTTCATCAGGAATTCGCGGGAACGCATGACGCCGAAACG  >  NZ_CP009273/214661‑214827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑