| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 892,811 | 0 | T | G | 61.5% | 6.9 / 12.4 | 13 | V91G (GTG→GGG) | potI | putrescine ABC transporter permease PotI |
| Reads supporting (aligned to +/- strand): ref base T (0/5); new base G (8/0); total (8/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.77e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.13e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTTAAGTCTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGATCCTCGGAACTATTGCGGCGGTGGTGCTGGTGCGCTTTGGCAGGTTTCGCGGATCAAATGGCTTTGCCTTTATGATCACCGCGCCGCTGGTAATGCCAGATGTCATCACGGGCTTGTCGC > NZ_CP009273/892737‑892900 | tttAAGTCTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGATCCTCGGAACTATTGCGGCGGTGGTGCTGGGGCGGTTTGGGAggct > 2:541215/1‑88 (MQ=255) gACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGATCCTCGGAACTATTGCGGCGGTGGGGCTGGGGCGCTTTGGCAGGGTTCGCGGGTc > 2:309628/1‑90 (MQ=255) gACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGATCCTCGGAACTATTGCGGCGGGGGGGGTGGGGGGGGTTGGCCGGGTTCCCgggac > 1:660850/1‑87 (MQ=255) gACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGATCCTCGGAACTATGGCGGCGGTGGGGCTGGGGGGCTTTTGGAGGTTTCGCGGGTc > 2:663456/1‑90 (MQ=255) gCCTGTGCGGCGACGGCGGCGGCGATCCTCGGAACTATTGCGGCGGTGGTGCTGGTGCGCTTTGGCAGGTTTCGCGGATCAAATGGCttt < 1:557664/90‑1 (MQ=255) gCCTGTGCGGCGACGGCGGCGGCGATCCTCGGAACTATTGCGGCGGGGGGGCTGGGGCGCTTTGGCAGGGTTCGCGGGTCAAAAGGCctt > 1:138598/1‑90 (MQ=255) gtgCGGCGACGGCGGCGGCGATCCTCGGAACTTTTGCGGCGGTGGTGCTGGTGCGCTTTGGCAGGTTTCGCGGATCAAATGGCTTTGCCt < 1:214484/90‑1 (MQ=255) gcgACGGCGGCGGCGATCCTCGGAACTATTGCGGCGGTGGTGCTGGTGCGCTTTGGCAGGTTTCGCGGATCAAATGGCTTTGCCTTTATg < 2:478839/90‑1 (MQ=255) gcgACGGCGGCGGCGATCCTCGGAACTATTGCGGCGGTGGGGCTGGGGCGCTTTGGCAGGGTTTGCGGGTCAAAAGGGTTTTCCTTTTTg > 2:721462/1‑90 (MQ=255) cgATCCTCGGAACTATTGCGGCGGTGGGGGTGGGGCGCTTTGGCAGGGTTCGCGGGTCAAAAGGGTTTGCCTTTTTGATCCCCCcgccgc > 1:124735/1‑90 (MQ=255) cTCGGAACTATTGCGGCGGTGGTGCTGGTGCGCTTTGGCAGGTTTCGCGGATCAAATGGCTTTGCCTTTATGATCACCGCGCCGCTGGTa < 1:249271/90‑1 (MQ=255) cTCGGAACTATTGCGGCGGTGGTGCTGGTGCGCTTTGGCAGGTTTCGCGGATCAAATGGCTTTGCCTTTATGATCACCGCGCCGCTGGTa < 2:17167/90‑1 (MQ=255) aTTGCGGCGGTGGTGCTGGGGCGCTTTGGCAGGTTTCGCGGATCAAATGGCTTTGCCTTTATGATCACCGCGCCGCTGGTAATGCCAGAt > 2:52385/1‑90 (MQ=255) ggtgcGCTTTGGCAGGTTTCGCGGATCAAATGGCTTTGCCTTTATGATCACCGCGCCGCTGGTAATGCCAGATGTCATCACGGGCTTGTc < 2:264227/90‑1 (MQ=255) gtgcGCTTTGGCAGGTTTCGCGGATCAAATGGCTTTGCCTTTATGATCACCGCGCCGCTGGTAATGCCAGATGTCATCACGGGCTTGTCg < 1:44816/90‑1 (MQ=255) tgcGCTTTGGCAGGTTTCGCGGATCAAATGGCTTTGCCTTTATGATCACCGCGCCGCTGGTAATGCCAGATGTCATCACGGGCTTGTCGc > 2:111698/1‑90 (MQ=255) | TTTAAGTCTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGATCCTCGGAACTATTGCGGCGGTGGTGCTGGTGCGCTTTGGCAGGTTTCGCGGATCAAATGGCTTTGCCTTTATGATCACCGCGCCGCTGGTAATGCCAGATGTCATCACGGGCTTGTCGC > NZ_CP009273/892737‑892900 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |