| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 906,370 | 0 | A | G | 56.2% | 8.2 / 19.9 | 16 | S46P (TCC→CCC) | poxB | ubiquinone‑dependent pyruvate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base A (0/7); new base G (8/1); total (8/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.40e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.80e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGTGGACATCCACTCGATGGTGCCCATGCGATTAAGACTGTCACTAAGACCGTTCAGAGAGTCGCCTGTGAC > NZ_CP009273/906284‑906436 | gCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGGGGGCAt > 2:591525/1‑90 (MQ=255) tCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGTGGACATCCACTc < 2:141364/90‑1 (MQ=255) gACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGGGGGCATGCACTCGGGGGTggc > 2:727417/1‑88 (MQ=255) gACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGGGGGCATCCCCCCCATgggggc > 1:205552/1‑86 (MQ=255) gCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGTGGACATCCACTCGATGGTGCCCATg < 2:593000/90‑1 (MQ=255) cGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGGGGGGATCCCCCCCATGGTGGCCCCCCGGTTAAGAc > 1:94109/1‑90 (MQ=255) cTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGTGGACATCCACTCGATGGTGCCCATGCGATTAAGACTg < 2:368948/90‑1 (MQ=255) cTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGTGGACATCCACTCGATGGTGCCCATGCGATTAAGACTg < 2:408762/90‑1 (MQ=255) gCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGGGGGCATCCCCTCGATGGGGGCCATGCGGTTTAGACTGTCACTAAGa > 2:146134/1‑90 (MQ=255) cTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGTGGACATCCACTCGATGGTGCCCATGCGATTAAGACTGTCACTAAGAc < 1:246159/90‑1 (MQ=255) aGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGGGGGCATCCCCCCCATGGGGGCCCCGCGATTAAAAACGGCACAAAGAACGtt > 1:306910/1‑90 (MQ=255) aGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGGGGGCATCCACTCCATGGTGCCCCTCCGGTTTAGAATGTCACTAAAACCGtt > 2:437734/1‑90 (MQ=255) aGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGGGGGCACCCCCCCCATGGGGCCCCCCCGCTTAAAACTGTCCCCAAAAcccct > 1:545725/1‑87 (MQ=255) ccAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGTGGACATCCACTCGATGGTGCCCATGCGATTAAGACTGTCACTAAGACCGTTCaga < 1:518909/90‑1 (MQ=255) cAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGTGGACATCCACTCGATGGTGCCCATGCGATTAAGACTGTCACTAAGACCGTTCagag < 2:451220/90‑1 (MQ=255) cgccCCTTCCTCGGGGGGGGGGGGAATCCCCTTGGTGGGGGCCATGCCATTAAGGCCGTTACTAAGACCGTTCAGAGAGTCGCCTGTGAc < 1:437734/90‑1 (MQ=255) | GCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGTGGACATCCACTCGATGGTGCCCATGCGATTAAGACTGTCACTAAGACCGTTCAGAGAGTCGCCTGTGAC > NZ_CP009273/906284‑906436 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |