| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 970,178 | 0 | A | G | 54.5% | 7.1 / 12.2 | 11 | E135G (GAG→GGG) | mukF | chromosome partition protein MukF |
| Reads supporting (aligned to +/- strand): ref base A (0/5); new base G (6/0); total (6/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.16e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GACTACTACATCCGTCAGCGCGAGTTTTCTACGCTGCGTCTTTCTATGCAGTTGTCGATTGTGGCGGGTGAGCTCAAACGCGCAGCAGATGCCGCCGAAGAGGGCGGTGATGAATTTCACTGGCACCGTAATGTCTATGCGCCAC > NZ_CP009273/970108‑970252 | gACTTCTACATCCGTCAGCGCGAGTTTTCTACGCTGCGTCTTTCTATGCAGTTGTCGATTGTGGCGGGGGGGGTCCAACCCGCCGCAGag > 1:261429/1‑89 (MQ=255) cAGCGCGAGTTTTCTACGCTGCGTCTTTCTATGCAGTTGTCGATTGTGGCGGGGGGGGGCCAACACCCCGCCGAAGCCGCCCCAGAgggg > 1:360531/1‑89 (MQ=255) tGCGTCTTTCTATGCAGTTGTCGATTGTGGCGGGTGAGCTCAAACGCGCAGCAGATGCCGCCGAAGAGGGCGGTGATGAATTTCACTGGc < 2:261429/90‑1 (MQ=255) ttCTATGCAGTTGTCGATTGTGGCGGGTGAGCTCAAACGCGCAGCAGATGCCGCCGAAGAGGGCGGTGATGAATTTCACTGGCACCGTaa < 2:306762/90‑1 (MQ=255) ttCTATGCAGTTGTCGATTGTGGCGGGTGAGCTCAAACGCGCAGCAGATGCCGCCGAAGAGGGCGGTGATGAATTTCACTGGCACCGTaa < 2:741490/90‑1 (MQ=255) aTGCAGTTGTCGATTGTGGCGGGGGGGGTCAAACGCGCAGCCGATGGCGCCGGAGAGGGGGGGGGGGGATTTTTCTTGCACCGGAATGtt > 2:229144/1‑89 (MQ=255) gTTGTCGATTGTGGCGGGGGGGGTCAAAAGCGCCGCAGAAGCCGCCGGAGAGGGGGGGGGGGAATTTTACTGGGACCCCAATGTTTAttc > 2:24129/1‑88 (MQ=255) tGTCGATTGTGGCGGGTGAGCTCAAACGCGCAGCAGATGCCGCCGAAGAGGGCGGTGATGAATTTCACTGGCACCGTAATGTCTATgcgc < 1:24129/90‑1 (MQ=255) tGTCGATTGTGGCGGGGGGGGTCAAAAGCGCCGCAGAAGGCGCCGGAGAGGGGGGGGGGGGATTTTACTTGGACCCGAATGTCTATtcgc > 2:257036/1‑90 (MQ=255) tGTCGATTGTGGCGGGGGGGGTCAAAAGCGCCGCAGAAGCCGCCGAAGAGGGGGGGGGGGAATTTTACTGGCACCCCAAAGTCTATtcgc > 2:248565/1‑90 (MQ=255) cGATTGTGGCGGGTGAGCTCAAACGCGCAGCAGATGCCGCCGAAGAGGGCGGTGATGAATTTCACTGGCACCGTAATGTCTATGCGCCAc < 1:61133/90‑1 (MQ=255) | GACTACTACATCCGTCAGCGCGAGTTTTCTACGCTGCGTCTTTCTATGCAGTTGTCGATTGTGGCGGGTGAGCTCAAACGCGCAGCAGATGCCGCCGAAGAGGGCGGTGATGAATTTCACTGGCACCGTAATGTCTATGCGCCAC > NZ_CP009273/970108‑970252 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |