Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273982,4700AC53.8% 6.0 / 14.0 13intergenic (‑32/+571)ompF/asnSporin OmpF/asparagine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (4/2);  new base C (0/7);  total (4/9)
Fisher's exact test for biased strand distribution p-value = 2.10e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.13e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TACCTGCTACTAACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTA  >  NZ_CP009273/982384‑982541
                                                                                      |                                                                       
tcccTGCTACTAACAGAGCAGGGACGATCCCTGCCAAAATATTGCGCTTCATCATTATTTATTCCCCTCAGGTTTTTTTTTATGACCCCt                                                                      <  1:697202/88‑1 (MQ=255)
     gCTACTAACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCAc                                                                 <  2:208543/90‑1 (MQ=255)
        cctaACAGAGCAGGGACGATCACTCCCAAAATATTGCGCTTCATCATTATTTATTACCCTCATGTTTTTTTTTATGACCCCTGCCACTGc                                                              <  1:828184/89‑1 (MQ=255)
           aaCAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTCCCCTCATGTTTTTTTTTATGACCCCTGCCACTGCCGt                                                           <  1:15673/90‑1 (MQ=255)
               gagCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAAt                                                       >  1:372607/1‑90 (MQ=255)
                                           gcgcTTCATCATTATTTATTCCCCTCATGTTTTTTTTTATGACCCCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAAc                           <  1:445712/90‑1 (MQ=255)
                                           gcgcTTCATCATTATTTATTCCCCTCATGTTTTTTTTTATGACCCCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAAc                           <  1:514339/90‑1 (MQ=255)
                                           gcgcTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACCCCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAAc                           <  2:722171/90‑1 (MQ=255)
                                                          ttattaCCCTCATGTTTTTTTTTATGACCCCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGttt            <  1:814008/90‑1 (MQ=255)
                                                            attaCCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTgg          <  2:372607/90‑1 (MQ=255)
                                                                  cTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCtt    >  1:248930/1‑90 (MQ=255)
                                                                  cTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCtt    >  2:340621/1‑90 (MQ=255)
                                                                    cATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTa  >  1:430866/1‑90 (MQ=255)
                                                                                      |                                                                       
TACCTGCTACTAACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTA  >  NZ_CP009273/982384‑982541

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑