| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,213,870 | 0 | A | C | 61.9% | 10.6 / 17.6 | 21 | T363P (ACC→CCC) | pdeG | cyclic diguanylate phosphodiesterase |
| Reads supporting (aligned to +/- strand): ref base A (6/2); new base C (0/13); total (6/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.16e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.82e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTTCATATTGGAATTAATTTTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCGG > NZ_CP009273/1213786‑1213947 | tttCATATTGGAATTAATTTTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACccgc > 1:828167/1‑90 (MQ=255) tattttGAAATTATTTTTGCCGTTTCGCATTTTTTTCCGCCGCCTTTTGTCGAGGAGTTTTTAATTTTCGGTGACAGTTTTACCcgccgc < 1:324128/86‑1 (MQ=255) cATTTTGAATTTATTTTTAGCCTTTCGCATTTTTTTCCGCCGCCTTTTGTCGACGAGTTTTTAAATTTCCGTGACATTTTTCCCcgccgc < 1:773000/90‑1 (MQ=255) aattttGAAATTATTTTTAGCGTTTCGCATATTTTTCCCCCGCCTTTTGTCGACGAGTTTTTAAATTTCCGTGACATTTTTCCCcgccgc < 1:201484/86‑1 (MQ=255) gtaattatttTTAGGCCTTCAAATTTTATTCCCCCGGTGTTTGCCGACAAGTGTTTAATTTTCGGAGCCATTTTTCCCCGCCGCGATTTa < 2:480919/88‑1 (MQ=255) taattTTAGCGTTTCGCATTTTTTTCCGCCGACTTTTGTCGACGAGTTTTAAATTTTCCGTGACAGTTTTCCCCGCCGCGATTTAAACCt < 2:543091/90‑1 (MQ=255) atttttAGCGCTTCGAATATTTTTCCGCCGACTTTTGTCGAGGAGTTTTTAATTTTCCGTGACATTTTTCCCCGCCGCGATTTAAACCtt < 2:492459/88‑1 (MQ=255) tAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCt > 2:568316/1‑90 (MQ=255) cTTCGCATTTTTTTCCGCCGACTTTTGTCGACGAGTTTTTAAATTTCCGTGACATTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAg < 1:149525/90‑1 (MQ=255) tCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAg > 1:256352/1‑90 (MQ=255) tCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAg > 1:514512/1‑90 (MQ=255) ttgTTTGTCGAGGAGTTTTTAATTTTCCGTGACTTTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTg < 1:386441/88‑1 (MQ=255) gTTTGTCGAGGAGTTTTTAAATTTCCGTGACATTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGaa < 1:175592/90‑1 (MQ=255) gTCGACGAGTTTTAAATTTTCCGTGACATTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGtt < 2:293416/90‑1 (MQ=255) tttAATTTTCCGTGACTTTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCt < 1:611518/90‑1 (MQ=255) ttCCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAg > 2:349255/1‑90 (MQ=255) tCCTTGACATTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGc < 1:568316/90‑1 (MQ=255) tCCGTGAAATTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGc < 2:39852/90‑1 (MQ=255) cccGTGACATTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGc < 1:84904/89‑1 (MQ=255) ccGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCg > 1:311355/1‑90 (MQ=255) cGTGACATTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCgg < 1:69911/90‑1 (MQ=255) | TTTCATATTGGAATTAATTTTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCGG > NZ_CP009273/1213786‑1213947 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |