| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,360,810 | 0 | A | G | 59.1% | 12.4 / 24.7 | 22 | G87G (GGA→GGG) | arnC | undecaprenyl‑phosphate 4‑deoxy‑4‑formamido‑L‑arabinose transferase |
| Reads supporting (aligned to +/- strand): ref base A (1/8); new base G (13/0); total (14/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.81e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.24e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTC‑ACGTTACTGGCGACTTAA‑TTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCC > NZ_CP009273/2360722‑2360883 | tGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGAt < 1:116399/90‑1 (MQ=255) ggTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGGtt > 1:446963/1‑90 (MQ=255) gAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGGTTTAGt > 2:37886/1‑90 (MQ=255) aaGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTc < 1:83889/90‑1 (MQ=255) gCGGAAAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTC‑ACGTTACTGGc < 1:785688/90‑1 (MQ=255) gagaACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGGTTTAGTC‑ACGTTACTgggggc > 1:70852/1‑86 (MQ=255) gagaACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGGGTTTGTC‑ACCGTACTGGCggc > 1:180780/1‑88 (MQ=255) agcAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTC‑ACGTTACTGGCGACTTa < 1:557345/88‑1 (MQ=255) aaCAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTC‑ACGTTACTGGCGACTTa < 2:237011/90‑1 (MQ=255) gCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGGTTTAGTC‑ACGTTTCTGGGGACTTTa‑ttt > 2:65084/1‑89 (MQ=255) gCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGGTTTAGTC‑ACCGTTCTGGGGGCCTAa‑tta > 1:194133/1‑90 (MQ=255) gtgtCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGGTTTAATC‑ACGTTTCTTGGGGCTTAATTTTTTACCCt > 2:765116/1‑90 (MQ=255) gtgtCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGAt < 1:817070/55‑1 (MQ=255) gtCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGGTTCAGTC‑ACCTTACTGGGGGCCTTA‑TTATTTTCCtttt > 1:216523/1‑88 (MQ=255) tATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGGGTTAGTCGACGTTA‑TGGGGGGTTAA‑TTTTTTACCTTGTTGc > 1:747216/1‑90 (MQ=255) ttttGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTC‑ACGTTACTGGCGACTTAA‑TTATTACCCTTGATGCCg < 1:65084/90‑1 (MQ=255) tttGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTC‑ACGTTACTGGCGACTTAA‑TTATTACCCTTGATGCCGa < 2:180780/90‑1 (MQ=255) tGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGGTTTAGTC‑ACCTTTCTGGGGGCTTTA‑TTATTTCCCCTTATGGCGGtc > 2:123949/1‑90 (MQ=255) aaTTACGGGCAACATTCAGCGATTATGGCGGGGTTTAGTC‑ACGTTACTGGCGACTTAA‑TTATTACCCCTGATGGCGGTCTTCCGAAACCg > 2:410248/1‑90 (MQ=255) cAACATTCAGCGATTATGGCGGGGTTGAGTG‑ACCTTACTGTCGACCTAA‑TTATTTTCCCTTTTTCCGATCTTCCCAATACGCCCgaagaa > 1:441266/1‑90 (MQ=255) cAACATTCAGCGATTATGGCGGGGATGAGGC‑ACCTTACTTGCGGCTTTA‑TTATTTACCTTTTTTTCGCTCTCCCGAAACCCCCAccagaa > 1:502372/1‑90 (MQ=255) cAGCGATTATGGCGGGATTCAGTC‑ACGTTACTGGCGACTTAA‑TTATTTCCCCTTATGCCGATCTCCAGAAACCCCCCGAAGAAAAccccc > 2:341926/1‑90 (MQ=255) | TGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTC‑ACGTTACTGGCGACTTAA‑TTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCC > NZ_CP009273/2360722‑2360883 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |