| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 353,586 | 0 | A | T | 57.1% | ‑2.7 / 12.3 | 14 | N272K (AAT→AAA) | cynR | transcriptional regulator CynR |
| Reads supporting (aligned to +/- strand): ref base A (5/1); new base T (0/8); total (5/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.00e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.20e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATA > NZ_CP009273/353497‑353643 | aGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTT‑CGCGGCGGCTGTCTGCCAGCTa > 2:33580/1‑90 (MQ=255) cTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTT‑CGCGGCGGCTGTCTGCCAGCTAttttt > 1:649929/1‑90 (MQ=255) cTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTT‑CGCGGCGGCTGTCTGCCAGCTAttttt > 1:670269/1‑90 (MQ=255) cTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTT‑CGCGGCGGCTGTCTGCCAGCTAttttt > 2:287453/1‑90 (MQ=255) cTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTT‑CGCGGCGGCTGTCTGCCAGCTAttttt > 2:442823/1‑90 (MQ=255) cacccGCGCTTTTACCCACCGCCATGTGAAAAAATGCCTT‑CGCGGCGGCTGTCTGCCAGCTTTTTTCCCGCCGCAAAAAAACCGCCGTtc < 1:87658/87‑1 (MQ=21) cacccGCGCATTTACCCACCCCCATGTGAAAAAATGCCTT‑CGGGGCGGCTGTCTGCCAGCTATTTTCCCGCCGCAACAAAACCGCCGTtc < 1:89076/87‑1 (MQ=25) cacccGCGCATTTACCCACCCCCATGTCCAAAAATGCTTC‑CGCGGCGGCTTTCTCCCAGCTTTTTTTCCCCCGCAAAAAAACCGCCGTtc < 1:444125/87‑1 (MQ=255) cacccGCCCTTTTATCCACCCCCATGTGCAAAAATGCCTT‑CGGGGGGGTTGTCTGCCAGTTTTTTTCCCGCCGCAACAAAACCGCCGTtc < 1:287453/87‑1 (MQ=21) gcgcATTTATCCAACGCCATGTGCAAAAATGCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTcc > 2:87658/1‑90 (MQ=255) cccAACGCCATGTGAAAAAATGCCTT‑CGGGGCGGCTGTCTGCCAGCTTTTTTCCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGc < 1:186736/89‑1 (MQ=37) cccATGTAAAAAAACCCCTC‑CGCGGCGGCTGTCTCCCAGTTTTTTTCCCCCCGCAAAAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCa < 1:354508/89‑1 (MQ=255) aaaaaTGCCTC‑CGCGGCGCCTGTCTGCCAGCTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATa < 1:281203/90‑1 (MQ=255) aaaaaCCCCTC‑CGCGGCGCCTGTCGGCCATTTTTTTTCCCCCCAAAAAAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATa < 2:617944/90‑1 (MQ=255) aaaaTTCCTTCCGGGGGGGCTTCCGGCCAGTTTTTTTCCCCCCGCAAAAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATa < 1:333525/90‑1 (MQ=255) | AGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATA > NZ_CP009273/353497‑353643 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |