Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273497,0200AC62.5% 2.6 / 15.9 16*559E (TAA→GAA) ybaLKef family K(+) transporter
Reads supporting (aligned to +/- strand):  ref base A (6/0);  new base C (0/10);  total (6/10)
Fisher's exact test for biased strand distribution p-value = 1.25e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.53e-04
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTC  >  NZ_CP009273/496941‑497108
                                                                               |                                                                                        
tctcGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTcacc                                                                                >  2:155793/1‑90 (MQ=255)
tctcGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTcacc                                                                                >  2:448320/1‑90 (MQ=255)
                      ggCGCTTCTTTTCATGCCAACTTTTTTTTCATTTTTTACTTCCGGCAACCCCCGTTTCCCCCGTCCCCACCTCCCCCGCCGGTGGCGttt                                                          <  1:295935/90‑1 (MQ=255)
                           tttttATCTTCCCTACTTTTTTTTAATTTTTTACACCCGGCACCCCCCTTTTCCCCCGCCCCCCCCCCCCCCGCCGGTGGCGTTTCcagc                                                     <  2:42117/87‑1 (MQ=255)
                           gTCTTATCATGCCTACATTTTTTTCATATTTTACACCCGGCAACCCCCGTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCcagc                                                     <  1:170369/90‑1 (MQ=255)
                           attttATCATTCTTATATTTTTTTCTTTTTTTACATCCGGCAACCTCCTTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCcagc                                                     <  1:367796/87‑1 (MQ=255)
                                    tcccTACATTTTTTTCATTTTTTACATCCGGCACCCCCCGTTTCCCCCGCCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGc                                            <  2:278064/88‑1 (MQ=255)
                                     gCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCa                                           >  1:241063/1‑90 (MQ=255)
                                     gCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCa                                           >  1:281695/1‑90 (MQ=255)
                                      ccTACATATTTTTCATTTTTCACACCCGGCACCCCCCTTTTCCCCCGCCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCAt                                          <  1:448320/90‑1 (MQ=255)
                                      ccTACATATTTTTAATATTTTACATCCGGCAACCCCCGTTTCCCCCGTCACCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCAt                                          <  1:155793/90‑1 (MQ=255)
                                                       ttttACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCAc                         >  2:77788/1‑90 (MQ=255)
                                                              cccGCCACCCCCCGTTTCCCCCGCCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGcc                  <  1:277890/89‑1 (MQ=255)
                                                               ccGCCACCCCCCGTTCCCCCCGCCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGccc                 <  2:374226/90‑1 (MQ=255)
                                                                        cctttttCCCCCGTCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTAcc        <  1:571555/86‑1 (MQ=255)
                                                                              tACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTc  >  1:391877/1‑90 (MQ=255)
                                                                               |                                                                                        
TCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTC  >  NZ_CP009273/496941‑497108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: