| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 497,036 | 0 | A | C | 58.8% | 10.2 / 19.3 | 17 | G553G (GGT→GGG) | ybaL | Kef family K(+) transporter |
| Reads supporting (aligned to +/- strand): ref base A (6/1); new base C (0/10); total (6/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.66e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.65e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACC > NZ_CP009273/496963‑497113 | ggCGCTTCTTTTCATGCCAACTTTTTTTTCATTTTTTACTTCCGGCAACCCCCGTTTCCCCCGTCCCCACCTCCCCCGCCGGTGGCGttt < 1:295935/90‑1 (MQ=255) tttttATCTTCCCTACTTTTTTTTAATTTTTTACACCCGGCACCCCCCTTTTCCCCCGCCCCCCCCCCCCCCGCCGGTGGCGTTTCcagc < 2:42117/87‑1 (MQ=255) gTCTTATCATGCCTACATTTTTTTCATATTTTACACCCGGCAACCCCCGTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCcagc < 1:170369/90‑1 (MQ=255) attttATCATTCTTATATTTTTTTCTTTTTTTACATCCGGCAACCTCCTTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCcagc < 1:367796/87‑1 (MQ=255) tcccTACATTTTTTTCATTTTTTACATCCGGCACCCCCCGTTTCCCCCGCCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGc < 2:278064/88‑1 (MQ=255) gCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCa > 1:241063/1‑90 (MQ=255) gCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCa > 1:281695/1‑90 (MQ=255) ccTACATATTTTTCATTTTTCACACCCGGCACCCCCCTTTTCCCCCGCCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCAt < 1:448320/90‑1 (MQ=255) ccTACATATTTTTAATATTTTACATCCGGCAACCCCCGTTTCCCCCGTCACCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCAt < 1:155793/90‑1 (MQ=255) ttttACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCAc > 2:77788/1‑90 (MQ=255) cccGCCACCCCCCGTTTCCCCCGCCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGcc < 1:277890/89‑1 (MQ=255) ccGCCACCCCCCGTTCCCCCCGCCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGccc < 2:374226/90‑1 (MQ=255) cctttttCCCCCGTCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTAcc < 1:571555/86‑1 (MQ=255) tACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTc > 1:391877/1‑90 (MQ=255) cGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGcacc > 2:331697/1‑90 (MQ=255) tCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCAt > 1:373441/1‑35 (MQ=255) ccACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCAt < 2:373441/34‑1 (MQ=255) | GGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACC > NZ_CP009273/496963‑497113 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |