Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273510,0800AC54.5% 8.7 / 12.5 11V79G (GTG→GGG) ybbJNfeD family protein
Reads supporting (aligned to +/- strand):  ref base A (5/0);  new base C (0/6);  total (5/6)
Fisher's exact test for biased strand distribution p-value = 2.16e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTC  >  NZ_CP009273/509998‑510156
                                                                                  |                                                                            
cGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACccgccg                                                                       >  1:410062/1‑90 (MQ=255)
      tcccaccaAAAAACGCCGCCCAATCAGCTGCCCCCCGCGCTGTTTAAAATGACTATCGCTGTGTTTTTTTCCGCCCCCCCGCCGCGACaa                                                                 <  2:194913/88‑1 (MQ=255)
         cAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCa                                                              >  2:320930/1‑90 (MQ=255)
                aaaCGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCATTTctgt                                                       >  1:602864/1‑87 (MQ=255)
                 aaCGTCGGCCAACCAGCTGCCCCCCGCGCTGGTTTAAATGCCTACCGCTGTGTTTTTGTTCGCGCCCCCGCCGCGACAACCATTTccacc                                                      <  1:35541/90‑1 (MQ=255)
                 aaCGTCGCCCAACCAGCTGCTGCCCGCGCTGTTTTAAATGACTATCGCTGTGTTTTTGTTCGCGCCCCCGCCGCGACAACCATTTccacc                                                      <  1:28103/90‑1 (MQ=255)
                       ggCCAACCAGCTGCCCCCCGCGGTGGTTAAAATGACTATCGCTGTGTTTTTGTTCGCCCCCCCGCCGCGACAACCATTTCCACCACAGcc                                                <  2:136941/90‑1 (MQ=255)
                                                  taaaaTGCCTCCCGCTTTGTTTTTGTCCCCCCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCaa                     <  2:256384/88‑1 (MQ=255)
                                                                  gtgCTTTTGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCa     >  1:55700/1‑90 (MQ=255)
                                                                    gCTTTTGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACt   >  1:580415/1‑90 (MQ=255)
                                                                     tttttGTCCCCCCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTc  <  2:410062/89‑1 (MQ=255)
                                                                                  |                                                                            
CGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTC  >  NZ_CP009273/509998‑510156

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑