| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,240,706 | 0 | C | A | 53.8% | 11.4 / 21.3 | 13 | H31N (CAT→AAT) | BW25113_RS06225 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (1/5); new base A (7/0); total (8/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.66e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGA > NZ_CP009273/1240617‑1240776 | tgATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGGTTTTGGTGGGCTTATTGAAc > 2:678036/1‑90 (MQ=255) ttAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTGCGCTTATTTAAAATTTAGAATTTATCAAACCt > 2:623246/1‑90 (MQ=255) tAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCtt < 1:364086/90‑1 (MQ=255) gTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTGCGCTTATTTAAAATTTAGAATTGATCAATCCTTCTGGCa > 2:84841/1‑90 (MQ=255) gTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTTTTTAAAATTTTGAATTGATTAATCCTTCTggga > 2:10431/1‑88 (MQ=255) gTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCa > 1:127026/1‑90 (MQ=255) gTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAAAATTTTGAAATGGTCAATGCTTTTTGCa > 1:353022/1‑90 (MQ=255) aGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTGCGCTTTTTGAAAATTTTGAATTTATCAATCCTTCTGGCAGATTAAGCCa > 2:505248/1‑90 (MQ=255) aaaaaTGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATAcc < 2:146627/90‑1 (MQ=255) aTGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGtt < 2:127026/90‑1 (MQ=255) gTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTc < 1:345122/90‑1 (MQ=255) gAGGCGGTTTTTGCTGCGCTTTTTTAAAATTTTGAATTGATCAATCCTTCTTGCAGATTAAGCCAAAAAAACCGTTCGGCAATGCTTTAg > 2:227956/1‑90 (MQ=255) gCGGTTTTTGCTGCGCTTATTGAAAATTTAGAATTGATCAATCCTTCTGGCAGATTAAACCAAAAAAACCGTTTCGCAAAGCTTTAGTTa > 2:303107/1‑90 (MQ=255) tttGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGa < 1:303107/90‑1 (MQ=255) | TGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGA > NZ_CP009273/1240617‑1240776 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |