| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,476,148 | 0 | T | C | 56.2% | 10.5 / 16.1 | 16 | F336S (TTC→TCC) | ynbD | phosphatase PAP2/dual specificity phosphatase family protein |
| Reads supporting (aligned to +/- strand): ref base T (6/1); new base C (0/9); total (6/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.74e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.98e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGACTGCTGGTGTTTATTTAGGGGCGTTTCCACGACATATTCCGGCACAGAATGCGGTTCTGGACGTCACCTTTGAATT‑CCCTCGCGGACGAGCCACAAAAGATCGACTCTATTTTTGTGTACCGATGCTGGATCTGGTGGTTCCGGAAGAGGGGGAGC > NZ_CP009273/1476070‑1476228 | tGACTGCGGGTGTTTATTTAGGGGCTTTTCCACGACATATCCCGGCACAGAATGCGGTTCTGGACGTCCCCTTTGAATC‑CCCTCGCGGAc < 1:96008/90‑1 (MQ=255) aCTGCTGGTGTTTATTTAGGGGCGTTTCCACGACATATTCCGGCACAGAATGCGGTTCTGGACGTCACCTTTGAATT‑CCCTCGCGGACGa > 2:421518/1‑90 (MQ=255) tgctgGTGTTTATTTAGGGGCGTTTCCACGACATATTCCGGCACAGAATGCGGTTCTGGACGTCACCTTTGAATT‑CCCTCGCGGACGAGc > 1:289852/1‑90 (MQ=255) gtTTATTTAGGGGCGTTTCCACGACATATTCCGGCACAGAATGCGGTTCTGGACGTCACCTTTGAATT‑CCCTCGCGGACGAGCCACaaaa > 1:72717/1‑90 (MQ=255) gTTTCCACGACATATTCCGGCACAGAATGCGGTTCTGGACGTCACCTTTGAATT‑CCCTCGCGGACGAGCCACAAAAGATCGACTCTAttt > 1:195548/1‑90 (MQ=255) ccATATTCCGGCAAAAAGTGGGTTTCGGGTCGCCCCTTTTGAATC‑CCCGGGGGGACGAGCCACAAAAGATCGACTCTATTTTTGTGTAcc < 1:570393/89‑1 (MQ=255) aCATATCCCGGCACAGAATGGGGTTCGGGACTTCCCCTTTGATTC‑CCCCCGCGGACGAGCCACAAAAGATCGACTCTATTTTTGTGTAcc < 1:577237/90‑1 (MQ=255) aCATATCCCGGCAAAGAAGGGGTTTCTGGACGTC‑CCTTTTAATTCCCCCCGGGGACGAGCCACAAAAGATCGACTCTATTTTTGTGTAcc < 2:76088/90‑1 (MQ=255) ttCCGGCACAGAATGCGGTTCTGGACGTCACCTTTGAATT‑CCCTCGCGGACGAGCCACAAAAGATCGACTCTATTTTTGTGTACCGATGc > 1:76088/1‑90 (MQ=255) tcccGCCAAAGAATGCGGTTCGGGCCGTCCCTTTTGAATC‑CCCTCGGGGACGAGCCACAAAAGATCGACTCTATTTTTGTGTACCGATGc < 2:72717/88‑1 (MQ=255) cccGGCAAAAAATGCGTTTCTGGACGTCCCTTTTGATTC‑CCCTCGCGGACGAGCCACAAAAGATCGACTCTATTTTTGTGTACCGATGCt < 1:283267/89‑1 (MQ=255) ccGGCACAGAAGGCGTTTCTGGACGTCTCCTTTGATTC‑CCCCGGCGGGGGAGCCACAAAAGATCGACTCTATTTTTGTGTACCGATGCTg < 2:536851/90‑1 (MQ=255) gCACAGAATGCGGTTCTGGACGTCACCTTTGAATT‑CCCTCGCGGACGAGCCACAAAAGATCGACTCTATTTTTGTGTACCGATGCTGGAt > 2:577237/1‑90 (MQ=255) aaTGCGGTTCTGGACGTCCCTTTTGATTC‑CCCCCGGGGAGGAGCCACAAAAGATCGACTCTATTTTTGTGTACCGATGCTGGATCtggtg < 2:541447/90‑1 (MQ=255) aaTGCGGTTCTGACCTCCTCTTTTGATTC‑CCCTGGGGGAGGAGCCACAAAAGATCGACTCTATTTTTGTGTACCGATGCTGGATCtggtg < 2:615765/90‑1 (MQ=255) ccTTTGATTC‑CCCTCGGGGAGGAGCCACAAAAGATCGACTCTATTTTTGTGTACCGATGCTGGATCTGGTGGTTCCGGAAGAGGGGGAGc < 2:55587/90‑1 (MQ=255) | TGACTGCTGGTGTTTATTTAGGGGCGTTTCCACGACATATTCCGGCACAGAATGCGGTTCTGGACGTCACCTTTGAATT‑CCCTCGCGGACGAGCCACAAAAGATCGACTCTATTTTTGTGTACCGATGCTGGATCTGGTGGTTCCGGAAGAGGGGGAGC > NZ_CP009273/1476070‑1476228 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |