| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,571,380 | 0 | A | C | 63.6% | 4.4 / 19.9 | 22 | V166G (GTA→GGA) | yddB | TonB‑dependent receptor plug domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base A (6/2); new base C (0/14); total (6/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.75e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.12e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGTTAACCAGTCCGAACGCGTAGTGCGATAACCCAATTTCACCTTGCTATCATCAGCGTTGAAGCGTTTGATCTTTGCATCAATTACCCCGCCATTGAAGCGACCAAATTCAACCGGCACAAAACTGTCATAAAGCGTCACATTGTCCAGTAAGCTGACATCAAGATAATA > NZ_CP009273/1571295‑1571465 | tGTTAACCAGTCCGAACGCGTAGTGCGATACCCCAATTCCCCCTTGCTATCATCAGCGTTGAAGCGTTTGATCTTTGCATCAATTCcccc < 2:482200/90‑1 (MQ=255)tGTTAACCAGTCCGAACGCGTAGTGCGATAACCCAATTTCACCTTGCTATCATCAGCGTTGAAGCGTTTGATCTTTGCATCAATTCcccc < 1:550919/90‑1 (MQ=255)tGTTAACCAGTCCGAACGCGTAGTGCGATAACCCAATTTCACCTTGCTATCATCAGCGTTGAAGCGTTTGATCTTTGCATCAATTAcccc < 1:406376/90‑1 (MQ=255) gTCCGAACGCGTAGTGCGATAACCCAATTTCACCTTGCTATCATCAGCGTTGAAGCGTTTGATCTTTGCATCAATTAcccc < 2:110235/81‑1 (MQ=255) gTCCGAACGCGTAGTGCGATAACCCAATTTCACCTTGCTATCATCAGCGTTGAAGCGTTTGATCTTTGCATCAATTAcccc > 1:110235/1‑81 (MQ=255) gTCCGAACGCGTAGTGCGATAACCCAATTTCACCTTGCTATCATCAGCGTTGAAGCGTTTGATCTTTGCATCAATTACCCCGCCATTGaa > 1:293971/1‑90 (MQ=255) gTCCGAACGCGTAGTGCGATAACCCAATTTCACCTTGCTATCATCAGCGTTGAAGCGTTTGATCTTTGCATCAATTACCCCGCCATTGaa > 1:315480/1‑90 (MQ=255) gATAACCCAATTTCACCTTGCTATCATCAGCGTTGAAGCGTTTGATCTTTGCATCAATTACCCCGCCATTGAAGCGACCAAATTCAACCg > 2:590691/1‑90 (MQ=255) aaCCCAATTTCACCTTGCTATCATCAGCGTTGAAGCGTTTGATCTTTGCATCAATTACCCCGCCATTGAAGCGACCAAATTCAACCGGca > 2:188350/1‑90 (MQ=255) aTTTCACCTTCCTATCATCAGGGTTGAAGCGTTTGATTTTTGCATAAATTCCCCCGCCATTGAAGCGACCAAATTCAACCGGCACAAAAc < 1:442036/90‑1 (MQ=255) ttccTATCATCAGCGTTGAAGCTTTTGATTTTTCCATCATTTCCCCCGCCATTGAAGCGACCAAATTCAACCGGCACAAAACTGTCATaa < 2:582916/87‑1 (MQ=255) ttGCTATCATCAGCGTTGAAGCTTTTGATCTTTGCATCAATTCCCCCGCCATTGAAGCGACCAAATTCAACCGGCACAAAACTGTCATaa < 1:616821/90‑1 (MQ=255) ttGCTATCATCAGCGTTGAAGCTTTTGATCTTTGCATCAATTCCCCCGCCATTGAAGCGACCAAATTCAACCGGCACAAAACTGTCATaa < 1:503734/90‑1 (MQ=255) gCTATCATCAGCGTTGAAGCGTTTGATCTTTGCATCAATTCCCCCGCCATTGAAGCGACCAAATTCAACCGGCACAAAACTGTCATAAAg < 1:20253/90‑1 (MQ=255) caGCGTTGAAGCTTTTGATCTTTGCATAAATTCCCCCGCCATTGAAGCGACCAAATTCAACCGGCACAAAACTGTCATAAAGCGTCACAt < 2:135843/90‑1 (MQ=255) caGCGTTGAAGCTTTTAATCTTTCCATAATTTCCCCCGCCATTGAAGCGACCAAATTCAACCGGCACAAAACTGTCATAAAGCGTCACAt < 2:315480/90‑1 (MQ=255) aGCTTTTGATCTTTGCATCAATTCCCCCGCCATTGAAGCGACCAAATTCAACCGGCACAAAACTGTCATAAAGCGTCACATTGTCCAGTa < 1:690628/90‑1 (MQ=255) ttGATCTTTGCATCAATTCCCCCGCCATTGAAGCGACCAAATTCAACCGGCACAAAACTGTCATAAAGCGTCACATTGTCCAGTAAGCTg < 2:36772/90‑1 (MQ=255) cTTTGCATCAATTCCCCCGCCATTGAAGCGACCAAATTCAACCGGCACAAAACTGTCATAAAGCGTCACATTGTCCAGTAAGCTGACATc < 1:158338/90‑1 (MQ=255) ttGCATCAATTACCCCGCCATTGAAGCGACCAAATTCAACCGGCACAAAACTGTCATAAAGCGTCACATTGTCCAGTAAGCTGACATCaa > 1:376699/1‑90 (MQ=255) aaaTTCCCCCGCCATTGAAGCGACCAAATTCAACCGGCACAAAACTGTCATAAAGCGTCACATTGTCCAGTAAGCTGACATCAAGataat < 2:531048/89‑1 (MQ=255) aaTTCCCCCGCCATTGAAGCGACCAAATTCAACCGGCACAAAACTGTCATAAAGCGTCACATTGTCCAGTAAGCTGACATCAAGataata < 1:335047/90‑1 (MQ=255) | TGTTAACCAGTCCGAACGCGTAGTGCGATAACCCAATTTCACCTTGCTATCATCAGCGTTGAAGCGTTTGATCTTTGCATCAATTACCCCGCCATTGAAGCGACCAAATTCAACCGGCACAAAACTGTCATAAAGCGTCACATTGTCCAGTAAGCTGACATCAAGATAATA > NZ_CP009273/1571295‑1571465 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |