Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,380,3570AT58.3% 2.6 / 13.5 12intergenic (+47/‑56)yfbL/yfbMM28 family metallopeptidase/YfbM family protein
Reads supporting (aligned to +/- strand):  ref base A (1/4);  new base T (7/0);  total (8/4)
Fisher's exact test for biased strand distribution p-value = 1.01e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.93e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCT  >  NZ_CP009273/2380277‑2380428
                                                                                |                                                                       
tGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAAAGACAAGCACATTTTTTTTTTCCTc                                                                >  1:18079/1‑90 (MQ=255)
  gtgtTATAATTTTGTTATACAACAGTAAATAAT‑AAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATc                                                             <  1:10521/90‑1 (MQ=255)
   tgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATc                                                             <  1:631017/90‑1 (MQ=255)
            tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAAAGACAAGCACATTTTTTTTTTCCTCATCTGtttttaa                                                    >  1:155004/1‑87 (MQ=255)
            tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAAAGACATGCACATTTTTTTTTTCCTCATTTGTTTTTAg                                                    >  1:16035/1‑90 (MQ=255)
            tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAAAGACAAGCACATTTTTTTTTTCCTCATCtttttttaa                                                    >  1:231070/1‑87 (MQ=255)
            tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAAAGAAAAGGACATTTTTTTTTTTCTCCTCtttttttat                                                    >  2:70654/1‑87 (MQ=255)
                          gTAAATAATAAAAATTTCTCGAGGCGGGTCCCGAAAAAAAGACAAGCACATTTTTTTTTTCCTCCTCTTTTTTTAGATGCGGAAAAAGaa                                      >  1:597437/1‑90 (MQ=255)
                                 ataaAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAAc                               <  1:435327/90‑1 (MQ=255)
                                 ataaAAATTTCTCGAGGCGGGGCCAGAAAAAACGACATGCACATTTTTTTTTCCCTCATCTGTTTTAAGATGCGAAAAATAAAAATCAAc                               >  1:638097/1‑90 (MQ=255)
                                              gAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAaaataa                  <  1:70654/90‑1 (MQ=255)
                                                              aaGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCt  >  2:103496/1‑90 (MQ=255)
                                                                                |                                                                       
TGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCT  >  NZ_CP009273/2380277‑2380428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑