| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,148,998 | 0 | T | G | 54.5% | 5.6 / 10.5 | 11 | G95G (GGT→GGG) | yqhD | alcohol dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (1/4); new base G (6/0); total (7/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.52e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.26e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCCAAACCCGGCTTATGAAACGCTGATGAACGCCGTGAAACTGGTTCGCGAACAGAAAGTGACTTTCCTGCTGGCGGTTGGCGGCGGTTCTGTACTGGACGGCA‑CCAAATTTATCGCCGCAGCGGCTAACTATCCGGAAAATATCGATCCGTGGCACATTCTGCAAACGGGCGGTAA > NZ_CP009273/3148911‑3149087 | gCCAAACCCGGCTTATGAAACGCTGATGAACGCCGTGAAACTGGTTCGCGAACAGAAAGTGACTTTCCTGCTGGCGGTTGGCGGCGGTTc < 1:262378/90‑1 (MQ=255)gCCAAACCCGGCTTATGAAACGCTGATGAACGCCGTGAAACTGGTTCGCGAACAGAAAGTGACTTTCCTGCTGGCGGTTGGCGGCGGTTc < 2:393783/90‑1 (MQ=255) aaaCCCGGCTTATGAAACGCTGATGAACGCCGTGAAACTGGTTCGCGAACAGAAAGTGACTTTCCTGCTGGCGGTTTGCGGGGGGTCttt > 2:32595/1‑88 (MQ=255) gCTTATGAAACGCTGATGAACGCCGTGAAACTGGTTCGCGAACAGAAAGTGACTTTCCTGCTGGCGGTTGGCGGCGGGTCTGTACTgggc > 1:697186/1‑88 (MQ=255) aCGCTGATGAACGCCGTGAAACTGGTTCGCGAACAGAAAGTGACTTTCCTGCTGGCGGTTGGCGGCGGGTCTGTACTGGGCGGGA‑CCaaa > 1:376864/1‑90 (MQ=255) gaACGCCGTGAAACTGGTTCGCGAACAGAAAGTGACTTTCCTGCTGGCGGTTGGCGGGGGGTTTGTACTGGGCGGGACCCAAAtttttgc > 2:538183/1‑86 (MQ=255) ggTTCGCGAACAGAAAGTGACTTTCCTGCTGGCGGTTGGCGGCGGGTCTGTACTGGGCGGCA‑CCAAATTTTTTCCCGCCGCGGGTAACTa > 2:145721/1‑90 (MQ=255) ttCGCGAACAGAAAGTGACTTTCCTGCTGGCGGTTGGCGGCGGTTCTGTACTGGACGGCA‑CCAAATTTATCGCCGCAGCGGCTAACTATc < 2:391010/90‑1 (MQ=255) cgcgAACAGAAAGTGACTTTCCTGCTGGCGGTTGGCGGCGGGTTTGTACTGGGCGGGA‑CCAAAATTTTTGCCGCCGCGGGTAAATATTCg > 2:229423/1‑90 (MQ=255) ttCCTGCTGGCGGTTGGCGGCGGTTCTTTACTGGGCGGCA‑CCAAATTTTTCCCCGCCGCGGCTAACTATCCGGGAAAAAACGAACCGCgg > 2:348858/1‑90 (MQ=255) ggcggcGGTTCTGTACTGGACGGCA‑CCAAATTTATCGCCGCAGCGGCTAACTATCCGGAAAATATCGATCCGTGGCACATTCTGCAAACg < 2:240145/90‑1 (MQ=255) ttCTGTACTGGACGGCA‑CCAAATTTATCGCCGCAGCGGCTAACTATCCGGAAAATATCGATCCGTGGCACATTCTGCAAACGGGCGGTaa < 1:229423/90‑1 (MQ=255) ttCTGTACTGGACGGCA‑CCAAATTTATCGCCGCAGCGGCTAACTATCCGGAAAATATCGATCCGTGGCACATTCTGCAAACGGGCGGTaa < 2:70805/90‑1 (MQ=255) | GCCAAACCCGGCTTATGAAACGCTGATGAACGCCGTGAAACTGGTTCGCGAACAGAAAGTGACTTTCCTGCTGGCGGTTGGCGGCGGTTCTGTACTGGACGGCA‑CCAAATTTATCGCCGCAGCGGCTAACTATCCGGAAAATATCGATCCGTGGCACATTCTGCAAACGGGCGGTAA > NZ_CP009273/3148911‑3149087 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |