| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,175,957 | 0 | A | T | 63.6% | ‑1.7 / 18.5 | 22 | I17F (ATT→TTT) | zupT | zinc transporter ZupT |
| Reads supporting (aligned to +/- strand): ref base A (3/5); new base T (14/0); total (17/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.13e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.12e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGC‑GGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTC‑T‑CGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATC > NZ_CP009273/3175887‑3176037 | gAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGC‑GGGGGGGGCCCCGTTTTTTTGGGCGGTTTTCTGCgg > 2:304602/1‑89 (MQ=255)gAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGC‑GGGGGGGGCCACGGTTTTTTGGGCGGTTTTCcgcgg > 1:505282/1‑89 (MQ=255)gAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGC‑GGGGGGAGCCACGTTTTTTTGGGCGGTTCTCGGCGt > 1:214694/1‑90 (MQ=255) gAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGGGGCCCCGTTTTTTGG‑GGGTTTTTCCGCGTTC‑TCCGGGCaaaa > 2:627753/1‑90 (MQ=255) gAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGC‑GGGGGCAGGCACGTTTTTTTGCGCGTTTTTCTGCGGTC‑T‑CGGGCaaaaa > 1:127310/1‑90 (MQ=255) aGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGC‑GGGGGGAGCCACGTTTTTTTGCGCGTTTCTTCGCGGTC‑T‑CGGGCcaaaaa > 2:240524/1‑89 (MQ=255) tGTCGATGTCAGTACCTCTCATTCTGACCATACTGGC‑GGGGGGGGCCCCGTTTTTTGTTGCGGTTTTCGGCGGTCTT‑TGGCCAAAAAccc > 1:453176/1‑90 (MQ=255) ctcATTCTGACCATACTGGC‑GGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTC‑T‑CGGGCAAAAAACCTCGAACCGCTTACTGGCg > 1:187318/1‑90 (MQ=255) cATTCTGACCATACTGGC‑GGGGGGAGCCCCGTTTTTTTGCGCGGTTTTCCGCGTTC‑T‑CGGGCAAAAAACCCCCAACCGCTTTCTGGCggt > 2:107763/1‑88 (MQ=255) cATTCTGACCATACTGGC‑GGGGGCAGCCACGTTTTTTGGCGCGGTTTTCGGCGTTC‑T‑CGGGCAAAAAACCTCGAACCCCTTACTGGCggt > 1:437282/1‑88 (MQ=255) ttCTGACCATACTGGC‑GGGGGGGGCCCCGTTTTTTTGGGCGGTTTTCTGCGTTC‑TCCGGGAAAAAACCCCCG‑ACCGCTTTCTTGCGgttt > 2:506656/1‑90 (MQ=255) gACCATACTGGC‑GGGGGCCGCCCCCTTTTTTTGCGCGGTTCTCGGCCTTC‑T‑CGGGCCAAAACCCCCGCACCGCCTACTGGCGgtttttct > 2:48882/1‑86 (MQ=255) gACCATACTGGC‑GGGGGCAGCCCCGGTTTTTTGGGCGTTTTTCGGCGTTT‑T‑CGGGCCAAAAACCCCGCACCGCTTTCTGGCGGTTTTGCt > 2:48883/1‑90 (MQ=255) gACCATACTGGC‑GGGGGCAGCCACGTTTTTTTGCGCGTTTTTCCGCGTTC‑T‑CGGGCAAAAAACCCCGAACCGCCTACTTGCGGTTTTGCt > 1:671394/1‑90 (MQ=255) gACCATACTGGC‑GGGGGCAGCCACGTTTTTTGGGGCGGTTTTCCGCGTTC‑T‑CCGGCAAAAAACCCCCAACCCCTTACTGGCGGTTTTGCt > 1:145194/1‑90 (MQ=255) aCCATACTGGC‑GGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTC‑T‑CGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTa < 2:453176/90‑1 (MQ=255) gggggCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTC‑T‑CGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTgcggc < 2:368161/90‑1 (MQ=255) ggggCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTC‑T‑CGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTgcggcg < 2:145194/90‑1 (MQ=255) gCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTC‑T‑CGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCgggg < 1:284749/90‑1 (MQ=255) aGCCACGTTTATTGGCGCGTTTCTCGGCGTTC‑T‑CGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGAt < 2:505282/90‑1 (MQ=255) gCCACGTTTATTGGCGCGTTTCTCGGCGTTC‑T‑CGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCggggggc > 2:402978/1‑87 (MQ=255) gCCACGTTTATTGGCGCGTTTCTCGGCGTTC‑T‑CGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGGTc > 2:59409/1‑90 (MQ=255) | GAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGC‑GGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTC‑T‑CGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATC > NZ_CP009273/3175887‑3176037 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |