Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	49,883	C→T	P21S (CCG→TCG)	folA →	type 3 dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	49,883	0	C	T	100.0%	80.2 / NA	27	P21S (CCG→TCG)	folA	type 3 dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/19);  total (8/19)

GACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAT  >  NZ_CP009273/49796‑49957                                                                                        |                                                                           gACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGTCg                                                                          >  2:112060/1‑90 (MQ=255)  aCAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGTCGt                                                                         <  1:240615/90‑1 (MQ=255)   cAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGTCGTg                                                                        >  2:232088/1‑90 (MQ=255)           tttATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGTCGTGGAAcctgc                                                                <  1:16214/90‑1 (MQ=255)               tCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGTCGTGGAACctgcctgc                                                            <  1:220921/90‑1 (MQ=255)               tCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGTCGTGGAACctgcctgc                                                            <  1:637964/90‑1 (MQ=255)                          cAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGTCGTGGAACCTGCCTGCCGATCTCGCCt                                                 <  2:740/90‑1 (MQ=255)                          cAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGTCGTGGAACCTGCCTGCCGATCTCGCCt                                                 <  2:236508/90‑1 (MQ=255)                                 cAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGTCGTGGAACctgcctgc                                                            >  1:601557/1‑72 (MQ=255)                                 cAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGTCGTGGAACctgcctgc                                                            <  2:601557/72‑1 (MQ=255)                                       gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGTCGTGGACCCTGCCTGCCGATCTCGCCTGGTTTAAACGCaa                                    <  2:684265/90‑1 (MQ=255)                                        aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGTCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCaa                                    >  2:357478/1‑89 (MQ=255)                                        aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGTCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAac                                   <  1:448228/90‑1 (MQ=255)                                        aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGTCGGGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAac                                   <  2:520516/90‑1 (MQ=255)                                        aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGAATGGAAAACGCCATGTCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCaa                                    <  1:357478/89‑1 (MQ=255)                                            cggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGTCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCt                               <  1:382059/90‑1 (MQ=255)                                            cggcgTTAGCGGTAGATCGCGTTACCGGCATGGAAAACGCCATGTCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCt                               <  1:438536/90‑1 (MQ=255)                                             ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGTCGTGGAACCTGCCTGCCGATCTCGCCTGGtt                                             <  2:93826/75‑1 (MQ=255)                                             ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGTCGTGGAACCTGCCTGCCGATCTCGCCTGGtt                                             >  1:93826/1‑75 (MQ=255)                                                 ttAGCGGTAGATCGCTTTATCGGCTTGGAAAACGCCATGTCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTtaaat                          <  2:452191/90‑1 (MQ=255)                                                        tAGATCGCGTTATCGGCATGGAAAACGCCATGTCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCg                   >  1:452840/1‑90 (MQ=255)                                                              gcgTTATCGGCATGGAAAACGCCATGTCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTa             >  2:109740/1‑90 (MQ=255)                                                                 ttATCGGCATGAAAAACGCCATGTCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATgg          <  2:296163/90‑1 (MQ=255)                                                                    tCGGCATGGAAAACGCCATGTCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgcc       <  1:119198/90‑1 (MQ=255)                                                                        cATGGAAAACGCCATGTCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCa   >  2:203825/1‑90 (MQ=255)                                                                         aggaaaacCGCCATGTCGTGAACCCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAt  <  2:181136/86‑1 (MQ=255)                                                                         aTGGAAAACGCCATGTCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAt  <  1:177246/90‑1 (MQ=255)                                                                                        |                                                                           GACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAT  >  NZ_CP009273/49796‑49957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑