| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 353,601 | 0 | C | A | 62.1% | 5.2 / 34.8 | 29 | L267F (TTG→TTT) | cynR | transcriptional regulator CynR |
| Reads supporting (aligned to +/- strand): ref base C (11/0); new base A (0/18); total (11/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.89e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.39e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGT‑GGCAATCGCTGCTGGTAACA > NZ_CP009273/353512‑353686 | tCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGcaac > 1:120684/1‑90 (MQ=255) cacccGCGTTTTTACCCACCCCCTTGTAAAAAAATCCCTC‑GGGGGCGGCTTTCGGCGAGTTTTTTTCCCGCCGCAAAAAAA‑CCGCCGTtc < 1:586105/87‑1 (MQ=255) cacccGCGTTTTTACCCACCCCCATGTAAAAAAAGCCCTC‑CGGGGCGGCTGTCGCCCATTTTTTTTCCCGCCGAAAAAAAA‑CCGCCGTtc < 1:183104/87‑1 (MQ=255) cacccGCGTTTTTACCCACCCCCATGT‑AAAAAATTCCTTCCGGGGGGGCTGTCTCCCATTTTTTTTCCCGCCGAAAAAAAA‑CCGCCGTtc < 1:228992/87‑1 (MQ=255) cacccGCGCTTTTACCCACCCCCATGTGCAAAAATGCCTT‑CGCGGCGGCTGTCTGCCAGCTTTTTTCCCGCCGCAAAAAAA‑CCGCCGTtc < 2:186740/87‑1 (MQ=21) ccGCGCTTTTACCCAACCCCATGGAAAAAAAGCCCTC‑CGGGGGGGCTTTCCCCCAGTTTTTTTCCCGCCGCAAAAAAA‑CCGCCGTtctct < 1:76393/90‑1 (MQ=255) ccGCGCATTTATCCAACGCCATGTGCAAAAATGCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAA‑CCGCCGTtctct > 1:712900/1‑90 (MQ=255) ccGCGATTTTACCAACCGCCATGGGAAAAATTGCTTG‑CGGGGGGGTTTTCTGCCAGCTTTTTTCCCGCCGAAAAAAAA‑CCGCCGTtctct < 2:407402/90‑1 (MQ=255) gcgcATTTATCCAACGCCATGTGCAAAAATGCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAA‑CCGCCGTTCTCTcc > 2:434850/1‑90 (MQ=255) gcgcATTTATCCAACGCCATGTGCAAAAATGCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAA‑CCGCCGTTCTCTcc > 2:205201/1‑90 (MQ=255) aTCCAACGCCATGTGCAAAAATGCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAA‑CCGCCGTTCTCTCCAGTAGTgg > 1:10679/1‑90 (MQ=255) aCGCCATGTGCAAAAATGCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAA‑CCGCCGTTCTCTCCAGTAGTGGCgggg > 1:528565/1‑90 (MQ=255) aCGCCATGTGCAAAAATGCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAA‑CCGCCGTTCTCTCCAGTAGTGGCgggg > 2:483220/1‑90 (MQ=255) aCGCCATGTGCAAAAATGCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAA‑CCGCCGTTCTCTCCAGTAGTGGCgggg > 1:178999/1‑90 (MQ=255) aCGCCATGTGCAAAAATGCCTT‑CGCGGAGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAA‑CCGCCGTTCTCTCCAGTAGTGGCgggg > 2:257204/1‑90 (MQ=255) gCCATGTGCAAAAATGCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAA‑CCGCCGTTCTCTCCAGTAGTGGCggggga > 1:38899/1‑88 (MQ=255) cccATGTAAAAAAATCCCTC‑CGGGGCGGCTGTCCGCCAGTTTTTTTCCCGCCGAAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCa < 2:712900/89‑1 (MQ=255) ccATGTAAAAAATTCCCTT‑CGGGGGGGCTTTCTGCCAGTTTTTTTCCCGCCAAAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCaa < 2:349585/90‑1 (MQ=255) aaaaaaCGCCTC‑CGGGGCGGCTGTCGGCCAGTTTTTTTCCCGCCAAAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAAt < 1:434850/89‑1 (MQ=255) aaaaTGCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGGGGGAGaaaaaa > 2:114067/1‑87 (MQ=38) t‑CGGGGCGGCTGCCTGCAAGTTTTTTTCCCCCCCAAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGcc < 2:676627/90‑1 (MQ=255) ccggggcggtTTTCTCCCAGTTTTTTTCCCCCCGAAAAAAAC‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGcc < 1:286569/86‑1 (MQ=255) ccggggcggcTGTCCCCCATTTTTTTCCCCCCCACAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGcc < 1:504402/86‑1 (MQ=255) ggggcTGTCTGCCATTTTTTTTCCCGCCAAAAAAAAC‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCa < 1:377783/88‑1 (MQ=255) gcTGTCTCCCAGTTTTTTTCCCCCCCCAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCAtgt < 2:82166/90‑1 (MQ=255) tttttcCCGCCGCAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGT‑GGCAATCgc < 2:19864/89‑1 (MQ=255) tttttcCCGCCGAAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGT‑GGCAATCgc < 2:278342/89‑1 (MQ=255) tttttcCCGCCAAAAAAAAC‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGT‑GGCAATCgc < 1:355552/89‑1 (MQ=255) tttttCCCCCCAAAAAAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGT‑GGCAATCgc < 1:114067/90‑1 (MQ=255) gcAACAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGGGAGAGAAAAAACTTTTAAACCCTCATGTTTTGTGGGCAAT‑GCTGCTGGGAACa > 2:118087/1‑90 (MQ=255) | TCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGT‑GGCAATCGCTGCTGGTAACA > NZ_CP009273/353512‑353686 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |