Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273542,2320GT55.6% 4.8 / 12.2 9V33F (GTT→TTT) fdrAacyl‑CoA synthetase FdrA
Reads supporting (aligned to +/- strand):  ref base G (3/1);  new base T (0/5);  total (3/6)
Fisher's exact test for biased strand distribution p-value = 4.76e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.17e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

ACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGA  >  NZ_CP009273/542143‑542296
                                                                                         |                                                                
aCGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGatgatg                                                                  >  2:587338/1‑90 (MQ=255)
    cTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTc                                                              <  2:425913/90‑1 (MQ=255)
                     gtTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTAcgc                                             >  1:529435/1‑90 (MQ=255)
                               ttCGGTAATTTTAATGATTTTTTCACAAAACCTCAGAAAATCAAAAAATGTTGATGTTTTTCCCGTAATGAGGGGTCCGCCCGCCAATaa                                   <  2:529435/90‑1 (MQ=255)
                                tCGGTAATTTTAATGATTATTTCACAAAAACTCAGCGAATCAAAAAATGTTGATGATTTTTCCGTAATGAGGGGTCCGCCCGCCAATaaa                                  <  2:97488/90‑1 (MQ=255)
                                  gttCATTTTAATTATTTTTTCAAAAAACCTCAGAAAAAAAAAAATTGTTGATGTTTTTTCCGTAATGGGGGGTCCGCCCGCCAATAAAGc                                <  1:287066/88‑1 (MQ=255)
                                      aGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGtta                            >  1:389474/1‑90 (MQ=255)
                                          tAATGATTTTTCCAAAAAACCTCAGCAAAAAAAAAAATTTTGATGTTTTTGCGGAAATGGTGGGTCCCCCCGCCAATAAAGCGTTATTAg                        <  1:180419/90‑1 (MQ=255)
                                                 tattTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACcac                 >  2:526664/1‑90 (MQ=255)
                                                                cAGCAAATAAAAAATTTTTGATGTTTTTTCCGTAATGATGGGTCCGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGa  <  2:691363/90‑1 (MQ=255)
                                                                                         |                                                                
ACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGA  >  NZ_CP009273/542143‑542296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑