| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 646,237 | 0 | C | T | 57.1% | 1.5 / 13.3 | 14 | M1M (ATG→ATA) † | citD | citrate lyase acyl carrier protein |
| Reads supporting (aligned to +/- strand): ref base C (2/4); new base T (8/0); total (10/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.50e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAAT > NZ_CP009273/646159‑646318 | cTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCt < 1:292913/90‑1 (MQ=255) tGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTTTTTTTTTTTTTTACCTg > 1:298153/1‑90 (MQ=255) gCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTTTTTTTATTTTTTACCTGt > 2:586058/1‑90 (MQ=255) gggCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGGTGGGTTATTTTTATTTTTTACCCGTTTCTCTTGCggg > 1:554786/1‑90 (MQ=255) gCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCggggg < 2:441466/90‑1 (MQ=255) gATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTTATTTTTTACCTGTTTCTCATGCggggggc > 2:400933/1‑88 (MQ=255) tCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTTATTTTTTCCCTGTTTTTCCTTCGGGGGGCTTTTTACGAGCTGCcg > 1:278735/1‑90 (MQ=255) ccAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTTACCTGTTTTTCATGCGGGGGGCTTTTGACGAGCTGCCGCGt > 1:265293/1‑90 (MQ=255) cAAGGGTGCCTGCAACGGCGGGCTGGGTTTTTTTTCTTTTTTACCTGTTTCTCCTGCGGGGGGCTTTTTACGAGCTGCCGCGTCCTGGCg > 1:662895/1‑90 (MQ=255) aGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCggg < 2:662895/90‑1 (MQ=255) gTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGt < 2:191409/90‑1 (MQ=255) cggcggGCTGGTTTATTTTTTTTTTTCCCCTTTTTCTCATGCGGGGGGCTTTTTACGGGCCGCCGCGTCCTTGCGGGGGTTCTCCAACAg > 1:446684/1‑90 (MQ=255) cggGCTGGTTTTTTTTTATTTTTTACCTGTTTCTCATTCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGGTCCAGCAgggt > 1:391113/1‑88 (MQ=255) tttATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAaat > 2:452519/1‑90 (MQ=255) cATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCa > 1:9052/1‑70 (MQ=255) cATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCa < 2:9052/70‑1 (MQ=255) | CTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAAT > NZ_CP009273/646159‑646318 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |