| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 799,325 | 0 | G | T | 63.6% | ‑2.6 / 10.8 | 11 | V123L (GTG→TTG) | ybhJ | hydratase |
| Reads supporting (aligned to +/- strand): ref base G (0/4); new base T (7/0); total (7/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.03e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.15e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCTCCGCATATTGCGGTCATCCATCAATATATGCGTGAGATGATGGCAGGCGGCGGCAAAATGATCCTCGGGTCAGAC > NZ_CP009273/799239‑799405 | actcactcTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGc < 1:121302/90‑1 (MQ=255) actcTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGACCCAGCGTTATGGCGGGATTTTTTTGCCTcc > 2:421699/1‑90 (MQ=255) ggcggcACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCTCCGCATATTGCGGTc < 2:488607/90‑1 (MQ=255) cggcACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCTCCGCATATTGCGGTcat < 1:496106/90‑1 (MQ=255) tgatgaCCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGGATTTTTTTGGCCCCCCCTATTGCGGGCATCCATCAATATATGCg > 2:318017/1‑90 (MQ=255) tgatgaCCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGGATTTTTTTGCCCCCCCCTATTTGGGGCATCCCTCAATATAAGCg > 2:440150/1‑90 (MQ=255) atgaCCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTTTGCCTCCCCATATTTCGGGCATCCCTCAATATATGCggg > 2:67048/1‑88 (MQ=255) tGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGGATTTTTTTGCCCCCCCATATTTCGGGCATCCCTCAATATATGGGTGAgatgat > 2:671356/1‑90 (MQ=255) ttGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTTTGCCTCCGCATATTGCGGGCATCCATCAATATATGCGGGGGATGATGGCAg > 2:252492/1‑90 (MQ=255) ggcCCAGCGTTATGGCGGTATTTTTTTGCCCCCCCATATTTCGGGCATCCCTCAATATATGCGTGGGATGATGGCAGGGGGGGGGaaaaa > 2:550000/1‑89 (MQ=255) ggTATTTTTGTGCCTCCGCATATTGCGGTCATCCATCAATATATGCGTGAGATGATGGCAGGCGGCGGCAAAATGATCCTCGGGTCAGAc < 1:67048/90‑1 (MQ=255) | ACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCTCCGCATATTGCGGTCATCCATCAATATATGCGTGAGATGATGGCAGGCGGCGGCAAAATGATCCTCGGGTCAGAC > NZ_CP009273/799239‑799405 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |