| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,213,865 | 0 | G | T | 58.3% | ‑0.8 / 12.6 | 12 | S361I (AGT→ATT) | pdeG | cyclic diguanylate phosphodiesterase |
| Reads supporting (aligned to +/- strand): ref base G (4/1); new base T (0/7); total (4/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.01e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.39e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCCGGAAGGTTTTCATATTGGAATTAATTTTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCGGT > NZ_CP009273/1213776‑1213948 | gCCGGAAGGTTTTCATATTGGAATTAATTTTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAg > 2:105201/1‑90 (MQ=255) ggAAGGTTTTCATATTGGAATTAATTTTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGttt > 1:676446/1‑90 (MQ=255) cATATTGAAATTATTTTTAGCGCTTCGCATTTTTTTTCGCCGCCTTTTGTCGACGAGTTTTTAATTTTCCGTGACATTTTTCCCcgccgc < 1:105201/90‑1 (MQ=255) gAATTAATTTTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCCGCCGCGATTTaa > 1:587592/1‑90 (MQ=255) tatttttAGCCCTTCGCATATTTTTTCCCCGCCGTTTGTCGACGAGTTTTTAATTTTCCGTGACATTTTTACCCGCCGCGATTTAAACCt < 1:590094/87‑1 (MQ=255) tAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCt > 1:4529/1‑90 (MQ=255) cTTCGAATTTTTTTTCGCCGTCGTTTGTCGACGGGTTTTTAATTTTCCGTGACATTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAg < 1:566272/90‑1 (MQ=255) cGCCGACGTTTGTCGAGGAGTTTTAAATTTTCCGTGACATTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGc < 1:77769/90‑1 (MQ=255) ttttGTGGACGAGTTTTTAATTTCCCGTGACATTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGaa < 1:158443/89‑1 (MQ=255) ttttGTCGAAGATTTTTTAATTTCCCGGGAAATTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGaa < 1:449782/89‑1 (MQ=255) gtTTAAATTTCCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTc > 1:81146/1‑90 (MQ=255) cGTGACATTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCgg < 2:429103/90‑1 (MQ=255) gTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCGGt < 2:81146/90‑1 (MQ=255) | GCCGGAAGGTTTTCATATTGGAATTAATTTTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCGGT > NZ_CP009273/1213776‑1213948 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |