| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,852,466 | 0 | T | G | 55.0% | 3.3 / 16.6 | 20 | V672G (GTG→GGG) | mutS | DNA mismatch repair protein MutS |
| Reads supporting (aligned to +/- strand): ref base T (7/2); new base G (0/11); total (7/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.64e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.51e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TATCGATCGCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACC‑TTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCT > NZ_CP009273/2852398‑2852555 | tATCGATCGCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACC‑TTTATGGTGGAGATGACTGAAACCGCCaa > 1:712664/1‑90 (MQ=255)tATCGATCGCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACC‑TTTATGGTGGAGATGACTGAAACCGCCaa > 1:66242/1‑90 (MQ=255) atcgatcgCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACC‑TTTATGGTGGAGATGACTGAAACCGCCAat > 2:573079/1‑90 (MQ=255) tccccGGGTGGGCGCGGCAGATGCCCGGGCGCCGGGGCGCTCAACT‑TTTGGGGGGGAGATGACTAAAACCGCCAATATTTTACATAACGc < 1:227282/88‑1 (MQ=255) tccccGGGTGGGCGCGGCAGAGGCCCTGGCCTCCGGGCCCTCACCT‑TTTATGGGGGAGATGACTAAAACCGCCAATATTTTACATAACGc < 2:478421/88‑1 (MQ=255) tccccGCGTGGGCGCGGCAGATGCCCGGGCGCCGGGGCGC‑CAACCTTTTAGGGTGGAGATGACTAAAACCGCCAATATTTTACATAACGc < 2:514632/88‑1 (MQ=255) tccccGCGGGGGCGCGGCAGATGCCCGGGCGCCGGGGCCCCCACCT‑TTTAGGGGGGAGATGACTAAAACCGCCAATATTTTACATAACGc < 2:548676/88‑1 (MQ=255) cccccGGGTGGGGGCGGCAGATGCCCGGGCGCCGGGGCGCTAACCT‑TTTAGGGGGGAGATGACTAAAACCGCCAATATTTTACATAACGc < 1:233559/88‑1 (MQ=255) cccGCGTGGGCGCGGCAGATGCCCTGGCGCCCGGGCGCTCACCC‑TTTATGGGGGAGATGACTAAAACCGCCAATATTTTACATAACGCCa < 2:295802/90‑1 (MQ=255) cgTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACC‑TTTATGGTGGAGATGACTGAAACCGCCAA‑ATTTTACATAACGCCACCGaa > 1:5097/1‑90 (MQ=255) gCAGATGACCTGGCGTCCGGGCGCTCAACC‑TTTATGGGGGAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTg < 2:39934/90‑1 (MQ=255) gCAGATGACCTGGCGCCGGGGCGCTCAACT‑TTTAGGGGGGAGATGACTAAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTg < 1:231897/90‑1 (MQ=255) cAGATGCCCGGGGGTCGGGGCCCTCACCT‑TTTGGGGGGGAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTgg < 1:25682/90‑1 (MQ=255) cccgggCGCCCGGGCCCTCACCC‑TTTATGGTGGAGATGACTAAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTaa < 2:226474/86‑1 (MQ=255) cGTCCGGGCGCTCAACC‑TTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATg > 2:270320/1‑90 (MQ=255) ccGGGCGCTCAACC‑TTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATgaga > 1:226474/1‑90 (MQ=255) gggCGCTCACCC‑TTTAGGGGGGAGATGACTAAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATc < 2:159674/90‑1 (MQ=255) gggCGCCCAACT‑TTTAGGGGGGAGATGTCAAAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATc < 1:448904/90‑1 (MQ=255) cAACC‑TTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTg > 2:418982/1‑90 (MQ=255) cc‑TTTATGGGGGAGATGAGTAAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGaa < 1:81965/90‑1 (MQ=255) tggAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCt > 2:547764/1‑90 (MQ=255) | TATCGATCGCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACC‑TTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCT > NZ_CP009273/2852398‑2852555 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |