| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,020,300 | 0 | A | C | 56.2% | 9.6 / 20.0 | 16 | E391D (GAA→GAC) | guaD | guanine deaminase |
| Reads supporting (aligned to +/- strand): ref base A (7/0); new base C (0/9); total (7/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.74e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.80e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAATTGTTCGTGATGATGACGTTG > NZ_CP009273/3020220‑3020381 | gggggAGCAAAATCTCGGGGCTTTGACGTTTTGATTGGCATTTTTTTCCCTGGAAAAGAGGTTGTTTTCGTGGTGATGGACCCCACCGcc < 1:155138/87‑1 (MQ=255)ggcggAGCAAAATCTCGGGGCCTTGACGATTTGATTGGCAACTTTTTCCCTGGCAAAGAGGCTGTTTTCGTGGTGAGGGACCCCACCGcc < 1:46672/90‑1 (MQ=255) ctctGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTAcagc > 2:262915/1‑90 (MQ=255) gggCTTTGACGATTTGATTGGCACTTTTTTCCCGGGAAAAGAGGCTGTTTTCGTGGTGATGGACCCCACCGCCACTCCGCTACAGCAGCt < 2:673413/90‑1 (MQ=255) gggCCTTGACGTTTTGTTTGCAAACTTTTTCCCTGAAAAAGAGGCTTTTTTGGGGGTGATGGACCCCACCGCCACTCCGCTACAGCAGCt < 1:430191/90‑1 (MQ=255) cTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTgcgc > 2:527278/1‑90 (MQ=255) ttttgtttgGCAATTTTTTCCCTGGAAAAGAGGCTGTTTTCGGGGTGAGGGACCCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACa < 2:491583/89‑1 (MQ=255) ttttGTTGGGCATTTTTTTCCCGGGAAAAGGGGTTGTTTTCGTGGTGATGGACCCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACa < 1:31433/89‑1 (MQ=255) aTTGGCATTTTTTTACCGGGAAAAGGGGCTGTTTTGGTGGTGATGGACCCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAActct < 1:219760/90‑1 (MQ=255) tACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCg > 1:688824/1‑90 (MQ=255) ggCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACaaa > 2:309510/1‑90 (MQ=255) ggCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACaaa > 2:3750/1‑90 (MQ=255) ggCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACaaa > 2:471324/1‑90 (MQ=255) ggCTGTTTTCGTGGTGATGGACCCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAATTGTTCGt < 2:305229/90‑1 (MQ=255) gtggtgATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAATTGTTCGTGATGATGACg > 2:643989/1‑90 (MQ=255) gtgATGAACCCCCCCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAATTGTTCGTGATGATGACGTTg < 1:318299/90‑1 (MQ=255) | GGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAATTGTTCGTGATGATGACGTTG > NZ_CP009273/3020220‑3020381 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |