| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,199,794 | 0 | C | T | 57.9% | 0.8 / 21.5 | 19 | intergenic (‑179/‑28) | ttdR/ttdA | DNA‑binding transcriptional activator TtdR/L(+)‑tartrate dehydratase subunit alpha |
| Reads supporting (aligned to +/- strand): ref base C (0/8); new base T (11/0); total (11/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.32e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-05 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAA > NZ_CP009273/3199706‑3199859 | gTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTcc < 1:446008/90‑1 (MQ=255) tCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACaa < 2:608351/90‑1 (MQ=255) cTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAATTTTTTTAGAaaaaaaaaaa > 1:488610/1‑90 (MQ=255) gTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAaaaaaaaaaaaa > 2:218704/1‑90 (MQ=255) tatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAAAAAAAAAAAAGGAGTTgg > 1:217843/1‑89 (MQ=255) tatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAAAAAAAAAAAAAAAAGGAGGTGc > 1:81294/1‑90 (MQ=255) tatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAATTTTTTCAGACAAAAAAAAAAAATGGAGGTGc > 2:295702/1‑90 (MQ=255) tatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAATTTTTTCAAAAAAAAAAAAAAAATGGAGGTgg > 1:31039/1‑89 (MQ=255) tatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGGAGTTTGTGGCGGGGAATTTTTTCAGACAAAAACAAAAAATGGAGGTGc > 2:117221/1‑90 (MQ=255) cACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAATTTTTTCAAACAAAAAAAAAAAAATGAGGTGCCatg > 1:213096/1‑90 (MQ=255) cGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCatgat < 1:458197/90‑1 (MQ=255) cGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCatgat < 2:489455/90‑1 (MQ=255) cacaTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACa < 1:117221/90‑1 (MQ=255) acaTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATGGAGTTGCCATGATGAGGGAAAGTAATAAACAACAg > 2:13041/1‑90 (MQ=255) aTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGc < 2:217843/90‑1 (MQ=255) aGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAAAAAAAAAAATGGAGGTGCCATGATGAGCGAAAATAATAAACAACAAGCAgggga > 2:531036/1‑86 (MQ=255) gTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAt < 1:73728/90‑1 (MQ=255) gTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAt < 2:189363/90‑1 (MQ=255) tGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATa < 1:520416/90‑1 (MQ=255) ggTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAAAAGGAGTTTCCCTGATTAGCGAAAGAAAAAAAAAAAAAGCAGGGAATaa > 2:394905/1‑90 (MQ=255) | GTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAA > NZ_CP009273/3199706‑3199859 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |