| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,401,195 | 0 | T | G | 57.9% | 11.2 / 18.9 | 19 | V77G (GTA→GGA) | panF | sodium/pantothenate symporter |
| Reads supporting (aligned to +/- strand): ref base T (2/6); new base G (11/0); total (13/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.03e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.32e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCTGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACAAT > NZ_CP009273/3401109‑3401283 | ggCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGGGCTGCTTTTAAATACGGGCTGGGCTGGGGAtt > 2:203917/1‑90 (MQ=255) ccGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCTGGGTATTGCTGGCGATGATTc < 2:564767/90‑1 (MQ=255) tataTCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCTGGGGATTGCTGGGGATGATTTAGCTTCCt > 2:179200/1‑90 (MQ=255) tatCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCTGGGTATTGCTGGCGATGATTCAGCTTCCTGc < 2:545143/90‑1 (MQ=255) gTGCCAGTTCGTTTATCGGCGGGGCAGGAGGGGGTTATAAAAAAGGGGTGGGGTGGGGGTTGCTGGGGGTGGTTTAGCCTCCTGCAGGCt > 1:138519/1‑90 (MQ=255) gTGCCAGTTCGTTTATCGGCGGGCCCGGGGGTGCTTTTAAATAAGGGCTGGGGGGGGGATTTGTGGGGGTGGTTTAGCTTCCTGCAGGCt > 1:592789/1‑90 (MQ=255) gTGCCAGTTCGTTTATCGGCGGGCCCGGGGCGGCTTTTAAAAACGGGGTGGGGTGGGGTTTTGTGGGGGTGGTTTAGCTTTCTTCCGTCt > 1:312439/1‑90 (MQ=255) gTGCCAGTTCGTTTATCGGCGGGCCAGGGGGTGCTTTTAAATAAGGGGTGGGGTGGGGGTTTCTGGGGGGGATTTAGCTTCCTGCAGTCt > 1:96755/1‑90 (MQ=255) gTGCCAGTTCGTTTATCGGCGGGCCAGGGGCTGCTTTTAAATAAGGGGTGGGGTGGGGATTTCTGGGGGTGGTTTAGCTTTCTGCCGTCt > 1:116692/1‑90 (MQ=255) gTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAAAACGGGCTGGGCTGGGGATTGCTGGCGATGATTCAGCTTCCTGCAGTCt > 2:178533/1‑90 (MQ=255) cAGTTCGTTTATCGGCGGGCCAGGAGGTGCTTATAAAAAAAGGCTGGGGTGGGGGTTTCTGGGGGTGATTCAGCTTCCTGCAGTGTTGCt > 1:701261/1‑90 (MQ=255) aGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCTGGGGATTTCTGGGGGTGGTTTAGCTTCCTGCAGTCTGGCtt > 2:82601/1‑90 (MQ=255) cGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCTGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCAc < 1:179200/90‑1 (MQ=255) gTTTATCGGCGGGCCAGGAGCTGCTTTTAAATAAGGGGTGGGGTGGGTGTTTGTGGGGGTGATTTAGCTTCCTTCAGCCTGGCttttcct > 1:460034/1‑86 (MQ=255) gggCCAGGAGCTGCTTATAAATAAGGGCTGGGGTGGGGATTGCTGGCGGTGGTTCAGCTTCCTGCAGGCTGGCTTTCACTCGGGATtctc > 1:173702/1‑90 (MQ=255) cGGGCTGGGCTGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTgc < 2:592789/90‑1 (MQ=255) tgggctgggTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGcgcc < 1:34111/90‑1 (MQ=255) ggctgggTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGcgccgc > 1:75568/1‑90 (MQ=255) gTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACAAt < 2:96755/90‑1 (MQ=255) | GGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCTGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACAAT > NZ_CP009273/3401109‑3401283 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |