| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,026,091 | 0 | T | G | 60.0% | 8.7 / 16.7 | 15 | G80G (GGT→GGG) | yigZ | IMPACT family protein |
| Reads supporting (aligned to +/- strand): ref base T (0/6); new base G (9/0); total (9/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.00e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.47e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCATTGCGTGGCGTGGGTCGCGGGTGCGCCGGATGATTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGTGGTAGTGCGCTACTACGGCGGCATATTG > NZ_CP009273/4026004‑4026172 | ccATTGCGTGGCGTGGGTCGCGGGTGCGCCGGATGATTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTaa < 2:278706/90‑1 (MQ=255) aTTGCGTGGCGTGGGTCGCGGGTGCGCCGGATGATTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGGaaaa > 1:350870/1‑89 (MQ=255) aTTGCGTGGCGTGGGTCGCGGGTGCGCCGGATGATTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGGAAAc > 2:640786/1‑90 (MQ=255) tggcgtggGTCGCGGGTGCGCCGGATGATTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGc < 1:582452/90‑1 (MQ=255) gcgGGTGCGCCGGATGATTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGGAAAACGATGCTCGCCCCGCTa > 2:269066/1‑90 (MQ=255) atTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCg < 1:365563/90‑1 (MQ=255) tctcAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGGAAAACCAAGCCCGCCCAGCTAAAGGGGAGCGGGGGCggg > 2:714294/1‑90 (MQ=255) gCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGGAAACCGATGCTCGCCCCGCTAATGGGCAGCGGCGTCGGGGAAATTAc > 2:199062/1‑90 (MQ=255) gCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGGAAACCGATGCTCCCCCAGCTAATGGGCAGCGGCGGCGGGGGAAATAc > 2:504894/1‑90 (MQ=255) gCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGGAAACCCATGCTCCCCCCCCTAAAGGGCAGCGGGGGCGGGGGAAATAc > 1:94126/1‑90 (MQ=255) gggTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGc < 1:640786/90‑1 (MQ=255) cgGGGAGCCGGCGGGAACGGCAGGGAAACCCATGCTCGCCCCGCTAATGGGGAGCGGCGGCGGGGGAAATACCCCTGTGGGAGGGGGcta > 2:500154/1‑90 (MQ=255) gAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGTGGTAGTGCGCtactac < 1:57688/90‑1 (MQ=255) gAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGTGGTAGTGCGCTACTACGGCGGCatat < 2:94126/90‑1 (MQ=255) aCGGCAGGGAAACCCATGCTCGCCCCGCTAATGGGCAGCGGCGGCGGGGGAAATACCCCTGTGGGAGGGGGCCACTACGGCGGGCTATTg > 1:41222/1‑90 (MQ=255) | CCATTGCGTGGCGTGGGTCGCGGGTGCGCCGGATGATTCTCAACAGCTGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGTGGTAGTGCGCTACTACGGCGGCATATTG > NZ_CP009273/4026004‑4026172 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |