| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,195,549 | 0 | G | T | 57.1% | ‑2.0 / 10.9 | 14 | S104* (TCA→TAA) | purD | phosphoribosylamine‑‑glycine ligase |
| Reads supporting (aligned to +/- strand): ref base G (3/3); new base T (8/0); total (11/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.49e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.80e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACGCCAGCGCAGGTTCTACCTCGGTGAAGTTCTGGTATTCCGCCGTAGGGATCTTATGGCGGGCCAGGAAATCTTTGGTAAACG‑CTTTTGA‑GCCTTCCAGTTGGGCCGCACCTGC‑GGTTGGGCCGAAGATTTTCAGCCCGGCGGCGCGGAAGGTATCGACCACGCCTTTCACCAGCGGC > NZ_CP009273/4195460‑4195638 | aCGCCAGCGCAGGTTCTACCTCGGTGAAGTTCTGGTATTCCGCCGTAGGGATCTTATGGCGGGCCAGGAAATCTTTGGTAAACG‑CTTTTg < 1:272519/90‑1 (MQ=255) cgcAGGTTCTCCCTCGGTGAAGTTCTGGTATTCCGCCGTAGGGATCTTATGGCGGGCCAGGAAATCTTTGGTAAACG‑CTTTTGA‑GCCTTc < 2:341202/90‑1 (MQ=255) cgcAGGTTCTACCTCGGTGAAGTTCTGGTATTCCGCCGTAGGGATCTTATGGCGGGGCCGGGAAAATTTTGTAAAAA‑CTTTTTT‑GCCTTc > 1:199957/1‑90 (MQ=255) cGGTGAAGTTCTGGTATTCCGCCGTAGGGATCTTATGGCGGGGCAGGGAAACTTTTGGGAAAG‑CTTTTTAGGCCTTCC‑GTTGGGgcgccc > 2:563665/1‑88 (MQ=255) cGGTGAAGTTCTGGTATTCCGCCGTAGGGATCTTATGGCGGACCAGGAAATCTTTGGTAAACG‑CTTTTGA‑GCCTTCCAGTTGGGCCGCAc > 1:227836/1‑90 (MQ=255) ggTATTCCGCCGTAGGGATCTTATGGCGGGCCAGGAAATCTTTTGTAAACG‑CTTTTTA‑GCCTTCCAGTTGGGCCGCACCTGC‑GGGTgggg > 2:221243/1‑89 (MQ=255) tCCGCCGTAGGGATCTTATGGCGGGCCAGGGAAACTTTTGTAAAAG‑CTTTTTA‑GCCTTCCAGTTGGGCCGCCCCCGC‑GGGTGGGGCggaa > 2:679925/1‑87 (MQ=255) tCCGCCGTAGGGATCTTATGGCGGGCCAGGGAAAATTTTGTTAAAG‑CCTTTTT‑GCCTTTCCCTTGGGGCGCCCCCCC‑GGGTGGGGCGGAg > 1:166525/1‑90 (MQ=255) tCCGCCGTAGGGATCTTATGGCGGGCCAGGAAAACTTTTGTAAAAGCCTTTTGG‑TCCTCCCATTTGGG‑CGCCCCCGCGGGTTGGGCGGag > 1:401049/1‑89 (MQ=255) gTAGGGATCTTATGGCGGGGCAGGGAATCTTTTGTTAAAG‑CTTTTTG‑GCCTTCCCGTTGGGGCGCGCCCCC‑GGTTGGGCCGCAGAatttt > 1:568895/1‑89 (MQ=255) gTAGGGATCTTATGGCGGGCCAGGAAATCTTTGGTAAACG‑CTTTTGA‑GCCTTCCAGTTGGGcc < 1:303225/63‑1 (MQ=255) gTAGGGATCTTATGGCGGGCCAGGAAATCTTTGGTAAACG‑CTTTTGA‑GCCTTCCAGTTGGGcc > 2:303225/1‑63 (MQ=255) tATGGCGGGCCAGGAAAACTTTTGTAAAAG‑CTTTTTA‑GCCTTCCCGTTGGGCCGCCCCCGC‑GGGTGGGGCGAAGAATTTTTGCCccgcgg > 1:286787/1‑90 (MQ=255) gggCCAGGAAATCTTTTGTAAACG‑CTTTTTA‑GCCTTCCCGTTGGGGCGCACCTGC‑GGGTGGGCCGGAGATTTTTAGCCCGGCGGCGCGGa > 1:232009/1‑90 (MQ=255) tttGGTAAACG‑CTTTTGA‑GCCTTCCAGTTGGGCCGCACCTGC‑GGTTGGGCCGAAGATTTTCAGCCCGGCGGCGCGGAAGGTATCGACCAc < 1:131518/90‑1 (MQ=255) gA‑GCCTTCCAGTTGGGCCGCACCTGC‑GGTTGGGCCGAAGATTTTCAGCCCGGCGGCGCGGAAGGTATCGACCACGCCTTTCACCAgcggc < 1:138892/90‑1 (MQ=255) | ACGCCAGCGCAGGTTCTACCTCGGTGAAGTTCTGGTATTCCGCCGTAGGGATCTTATGGCGGGCCAGGAAATCTTTGGTAAACG‑CTTTTGA‑GCCTTCCAGTTGGGCCGCACCTGC‑GGTTGGGCCGAAGATTTTCAGCCCGGCGGCGCGGAAGGTATCGACCACGCCTTTCACCAGCGGC > NZ_CP009273/4195460‑4195638 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |