| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,368,619 | 0 | A | C | 57.1% | 12.7 / 26.5 | 21 | V48G (GTA→GGA) | ampC | BlaEC family class C beta‑lactamase |
| Reads supporting (aligned to +/- strand): ref base A (9/0); new base C (0/12); total (9/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.40e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.41e-05 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCC‑TGATAAA‑TT‑ACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCA > NZ_CP009273/4368534‑4368708 | aaaCAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCC‑TGATAAA‑TT‑accgc > 1:175778/1‑90 (MQ=255)aaaCAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCC‑TGATAAA‑TT‑accgc > 2:232686/1‑90 (MQ=255) tGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCC‑TGATAAA‑TT‑ACCGCCACCGCCATa > 2:675304/1‑90 (MQ=255) tgtgACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCC‑TGATAAA‑TT‑ACCGCCACCGCCATACCCgg > 1:352683/1‑90 (MQ=255) gtgACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCC‑TGATAAA‑TT‑ACCGCCACCGCCATACCCggg > 2:134536/1‑90 (MQ=255) aCGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCC‑TGATAAA‑TT‑ACCGCCACCGCCATACCCGGGATc > 1:460193/1‑90 (MQ=255) gggCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCC‑TGATAAA‑TT‑ACCGCCACCGCCATACCCGGGATCtt > 2:135236/1‑90 (MQ=255) ttttttGGCGATCCCCCCATACCCCCGGGAAAAGTAATAAGGTTTCCCC‑TAATAAA‑TT‑CCCGCCACCGCCATACCCGGGATCTTttgttg < 2:12952/89‑1 (MQ=255) tttttGGCGGTCCCCCCATCCCCCGGGGAAAAATAATATGTTTTCCCC‑TAAAAAA‑TT‑CCCGCCCCCGCCATACCCGGGATCTTTTGTTGc < 1:351397/90‑1 (MQ=255) ttttgGGCGATCCCCCCATA‑CCCCAAGAAAAAAAAAAATGTTTTCCC‑TTAAAAATTT‑CCCCCCCCCGCCATACCCGGGAACTTTTGTTGc < 1:445406/90‑1 (MQ=255) atccccGCATCGCCCAAGGAAAAGAAATATTTTTTCCCT‑TAAAAAA‑TC‑CCCGCCCCCGCCATACCCGGGATCTTTTGTTGCTCTATAAGc < 1:350909/86‑1 (MQ=255) aTGCCCGCATACCCCCAGGAAAAAAAATAAGTTTTCCCC‑TAAAAAA‑TC‑CCCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGc < 1:102746/90‑1 (MQ=255) ccccccAGGAAAAGAAATAGGGTTTCCCC‑TAAAAAA‑TT‑CCCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATtg < 2:175778/88‑1 (MQ=255) gCCCCAGGTAAAGTAATAAGGTTTACCC‑TGATAAA‑TT‑ACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATtgt > 1:449849/1‑90 (MQ=255) cccGGGAAAAGTAATAAGTTTTCCCC‑TAAAAAA‑TT‑CCCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGc < 2:90582/90‑1 (MQ=255) ccAGGTAAAGTAATAAGGTTTACCC‑TGATAAA‑TT‑ACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCg > 2:704705/1‑90 (MQ=255) ccAGGAAAAATAAAAAAGTTTTCCCTTAAAAAA‑TT‑CCCCCCCCCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGc < 2:319498/90‑1 (MQ=255) cgggTAAAGAAATAATTTTTCCCT‑TGAAAAT‑TT‑CCCGCCCCCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGa < 2:352683/88‑1 (MQ=255) gagTAAAATAATTATGTTTTCCC‑TTAAAAA‑TTCCCCGCCCCCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGa < 1:696384/88‑1 (MQ=255) aaaGAAATAAGTTTTCCCC‑TAAAAAA‑TT‑CCCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGcac < 2:283099/90‑1 (MQ=255) ttACCC‑TGATAAA‑TT‑ACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGAtt > 1:580158/1‑90 (MQ=255) tcccc‑TAAAAAA‑TC‑CCCCCCCCCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGAttt < 2:101464/88‑1 (MQ=255) ccc‑TGATAAA‑TT‑ACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATttgt > 2:517441/1‑90 (MQ=255) accgccaccgccATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGgcagca > 1:3032/1‑90 (MQ=255) | AAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCC‑TGATAAA‑TT‑ACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCA > NZ_CP009273/4368534‑4368708 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |