| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 49,884 | C→T | P21L (CCG→CTG) | folA → | type 3 dihydrofolate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 49,884 | 0 | C | T | 96.3% | 74.2 / ‑4.9 | 27 | P21L (CCG→CTG) | folA | type 3 dihydrofolate reductase |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (9/17); total (10/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.70e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01 | |||||||||||
AATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGA > NZ_CP009273/49799‑49965 | aaTTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTgg > 2:616589/1‑90 (MQ=255) aaTGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTg < 2:661349/90‑1 (MQ=255) aaTGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTg < 2:453974/90‑1 (MQ=255) tCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTa < 1:616589/90‑1 (MQ=255) cAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTg < 1:37148/84‑1 (MQ=255) cAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTg > 2:37148/1‑84 (MQ=255) cAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTaa > 2:77749/1‑90 (MQ=255) cAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTaa > 2:691668/1‑90 (MQ=255) tGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCa < 2:211272/90‑1 (MQ=255) gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCaa < 1:85879/90‑1 (MQ=255) gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCaa < 1:50514/90‑1 (MQ=255) aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAac < 1:481540/90‑1 (MQ=255) ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGTCGTGGAACCTGCCTGCCGATCTCGCCTGGGTTAAACGCAACACCtt > 1:575694/1‑90 (MQ=255) ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGcc > 1:110854/1‑70 (MQ=255) ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGcc < 2:110854/70‑1 (MQ=255) tAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGACCTCGCCTGGTTTAAACACAACACCTtaaata < 2:545593/90‑1 (MQ=255) aGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTtaaataa < 2:659170/90‑1 (MQ=255) aGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGt < 2:610622/90‑1 (MQ=255) aGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGt < 2:637165/90‑1 (MQ=255) gcgTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTa > 1:556103/1‑90 (MQ=255) gcgTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTa > 2:693356/1‑90 (MQ=255) ttATCGGCATGGGAAACGCCATGCTGTGGAACCTGCCCGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATgg > 2:627667/1‑90 (MQ=255) aTGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAt < 2:677919/90‑1 (MQ=255) gggAAACCGCCATGCTGTGGAACCTCCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATa < 1:147373/89‑1 (MQ=255) aaaaCGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCt < 2:556103/90‑1 (MQ=255) cGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGa > 1:381651/1‑90 (MQ=255) cGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGa < 1:77749/90‑1 (MQ=255) | AATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGA > NZ_CP009273/49799‑49965 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |