| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,316,733 | 0 | T | C | 55.6% | 11.7 / 20.7 | 18 | G157G (GGA→GGG) | trpE | anthranilate synthase component I |
| Reads supporting (aligned to +/- strand): ref base T (6/2); new base C (0/10); total (6/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.51e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.11e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGAT > NZ_CP009273/1316658‑1316809 | cATCGGCTTTTCAGGGAAAAAAAAAAAGAAATCAGGGCATTTTTTTTCCGCTGACATTTGGGGTAAATCTTAAAACCCCGCCACAAGGTc < 2:191673/90‑1 (MQ=255)cATCGGCTTTCCAGCGAAAAAAAAACAAAAATCGGGGCATTTTTTTCCCGCTGACAGTTGGGGAAAATCTTAAAACCCCGCCACAAGGTc < 2:49724/90‑1 (MQ=255)cATCAGCTTTTCAGCGAAAAAAAAAAAGAAATCAGGGCAGTTTTTTTCCGCTGACAGTTGGGGTAATTCTTCAAATCCCGCCACAAGGTc < 1:282064/90‑1 (MQ=255) aGCGTTTCAGCGAAATAAAAACAAAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGAAAATCTTCAAACCCCGCCACAAGGTCATaa < 1:369913/90‑1 (MQ=255) ttCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCATAAGAGaa > 1:896792/1‑90 (MQ=255) cGAGATAAAAACAGAAATCAGGGCAGTTTTTTTCCGCTGACAGTTGCGGTAAATCTTCAAACCCCGCCACAAGGTCATAAGAGAACAGgc < 1:230408/90‑1 (MQ=255) aaaaacaaaaacCGGGGCATTTTTTTCCCGCTGAAAGTGGCGGAAATTTTTAAAACCCCGCCACAAGGTCATAAGAGAACAGGCCGCCga < 2:228632/90‑1 (MQ=255) aaTCAGGGCATTTTTTTCCCGCTGACAGTTGCGGAAAATCTTAAAACCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATgg < 2:541079/90‑1 (MQ=255) gggCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTtct > 1:636209/1‑90 (MQ=255) gTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCTCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGttc > 2:251822/1‑90 (MQ=255) tttttcCCGCTGACAGTTGCGGAAAATTTTAAAACCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGttctt < 1:83265/88‑1 (MQ=255) tcccGCTGACAGTTGCGGAAAATCTTAAAACCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTcttcctt < 1:317471/88‑1 (MQ=255) cccGCAGACAGTGGCGAAAAATCTAAAACCCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTcttccttc < 2:636209/89‑1 (MQ=255) aCAGTTGCGGTAAATCTTAAAACCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACAt < 1:58883/90‑1 (MQ=255) ttGCGGTAAATCTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCaa > 2:657223/1‑90 (MQ=255) gTAAATCTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGAt > 1:1250406/1‑90 (MQ=255) aaaTCTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCtt > 1:772225/1‑58 (MQ=255) aaaTCTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCtt < 2:772225/58‑1 (MQ=255) | CATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGAT > NZ_CP009273/1316658‑1316809 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |