| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,819,318 | 0 | G | A | 50.0% | 8.7 / 14.4 | 20 | intergenic (‑124/+79) | ves/spy | environmental stress‑induced protein Ves/ATP‑independent periplasmic protein‑refolding chaperone Spy |
| Reads supporting (aligned to +/- strand): ref base G (9/1); new base A (0/10); total (9/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.19e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTA‑CAACTGTTTG‑AGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATT > NZ_CP009273/1819230‑1819401 | tCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTA‑CAACTGTTTG‑AGAAGagaaaaga > 1:166183/1‑90 (MQ=255) gTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTA‑CAACTGTTTG‑AGAAGAGAAAAGAAAACCGCCGATCCTGTc > 2:903294/1‑90 (MQ=255) ttATCGGCACCGGGGGTGATTTACTTTGGTGGTGATCAGGTTTTTTTTA‑CAACTTTTTG‑AGAAAAAAAAAAAAAACCGCCGATCCTGTcc < 2:78958/90‑1 (MQ=255) tCGGCACCTGGGGTATTTTACTTTGGTGGTGATCAGGTTTTTTTTA‑AAATTGTTTG‑AGAAGAAAAAAAAAAACCGCCGATCCTGTCCAcc < 1:729197/90‑1 (MQ=255) tCGGAACCTGGGTTGTTTTACTTTGGGGGTGACCAGTTTTTTTTTA‑CATTTGTTGG‑AAAAAAAAAAAAAAACCCGCCGATCCTGTCCAcc < 2:1126235/90‑1 (MQ=255) tCGGAACCTGGGGTGATTTACCTGGGTGGTG‑TCAGGGTTTTTTTC‑AAAGTTTTTGAAGAAGAAAAAAAAAAACCGCCGATCCTGTCCAcc < 2:642142/90‑1 (MQ=255) cAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTA‑CAACTGTTTG‑AGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCAt > 2:13066/1‑90 (MQ=255) aaCTGGGGTGATTTACGTTAGTGGTAATCAGGTTTTTTTTA‑AAACTGTTTG‑AGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCAtt < 1:624220/90‑1 (MQ=255) gttaTTTACGTTAGGGGTGATCGGTTTTTTTTTA‑CATTTTTTGG‑AAAAAAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCaa < 2:769768/87‑1 (MQ=255) gTGATTTACGTTAGTGGTGATC‑GGTTTTTTTTACCAACTTTTTG‑AAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCaa < 2:1037508/90‑1 (MQ=255) ttACGTTAGTGGTGATCAGGCTTTTTTTA‑CAACTGTTTG‑AGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAg > 2:400605/1‑90 (MQ=255) gTTAGTGGTGATCAGGCTTTTTTTA‑CAACTGTTTG‑AGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGa > 2:239878/1‑90 (MQ=255) gtggtgATCAGGCTTTTTTTA‑CAACTGTTTG‑AGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCaa < 1:300706/77‑1 (MQ=255) gtggtgATCAGGCTTTTTTTA‑CAACTGTTTG‑AGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCaa > 2:300706/1‑77 (MQ=255) gATCAGGCTTTTTTTA‑CAACTGTTTG‑AGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcgg > 2:227660/1‑90 (MQ=255) tttttA‑CAACTGTTTG‑AGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCGTAAg > 1:1001607/1‑90 (MQ=255) a‑CAACTGTTTG‑AGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGttttt > 2:1095026/1‑90 (MQ=255) gTTTG‑AGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTg > 1:608681/1‑90 (MQ=255) g‑agaagaaaaAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAg < 1:53468/90‑1 (MQ=255) g‑agaagaaaaAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAg < 1:249329/90‑1 (MQ=255) aaagaaaaAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGat < 2:166183/89‑1 (MQ=255) agaaaagaaaACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGattatt > 1:1263157/1‑90 (MQ=255) | TCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTA‑CAACTGTTTG‑AGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATT > NZ_CP009273/1819230‑1819401 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |