| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,380,341 | 0 | G | A | 48.0% | 16.4 / 17.9 | 25 | intergenic (+31/‑72) | yfbL/yfbM | M28 family metallopeptidase/YfbM family protein |
| Reads supporting (aligned to +/- strand): ref base G (2/11); new base A (12/0); total (14/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.02e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCAT‑CTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCT > NZ_CP009273/2380261‑2380428 | tGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGca < 1:620335/90‑1 (MQ=255)tGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGca < 1:702198/90‑1 (MQ=255) cAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCCGAAAAAAAGACAAGGACattt > 1:732629/1‑90 (MQ=255) cAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAAAGACAAGCACattt > 1:362827/1‑90 (MQ=255) aGTGGATGGTGTTATAACTTTGTTATATAACAGTAAATAATAAAAATTTCCCGAGGCGGGTCCAGAAAAAGCGACATGCACattttattt < 1:1226485/90‑1 (MQ=255) gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCAt < 1:381563/90‑1 (MQ=255) tgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCAT‑c < 2:1268979/90‑1 (MQ=255) tgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCAT‑c < 2:1104125/90‑1 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCCCAAAAAAAGACATGCACATTTTTTTTTTCCTCCT‑Ctttttttaa > 1:1116977/1‑87 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAAAAAAAACGACAAGCACATTTTTTTTTTCCTCAT‑Ctttttttaa > 1:637426/1‑87 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCGGAAAAAAACACAAGCACAATTTATTTTTTCTCCT‑CTTTTTTata > 2:1045052/1‑88 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAAAGACAAGCACATTTTTTTTTTCCTCCT‑Ctttttttaa > 1:1265197/1‑87 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAAAAAAAAGAAAAATTTATTTTTTCTCATCCTGTTTTaa > 2:622467/1‑90 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCACAAAAAAAAACAAGGACATTTTTTTTTTCCTCTT‑Cttttttttt > 2:1122378/1‑87 (MQ=255) tGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAAAGACAAGCACATTTTTTTTTTCCTCAT‑CTTTTTTTAGAt > 1:632106/1‑90 (MQ=255) caacaGTAAATAATAAAAATTTCTCGAGGCGGGGCCTGAAAAAAAGAAAAACAAAATTTATTTTTTCCCCT‑CTTTTTTTAAATGAGaaaa > 2:630015/1‑89 (MQ=255) gTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAACGACATGCACATTTTTTTTTTCCTCCT‑TTTTTTTTAAATGGGAAAAATTaa > 1:526946/1‑90 (MQ=255) aaaaaTTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCAT‑CTGTTTTAAGATGCGAAACATGAAATTCAACgg < 2:541473/90‑1 (MQ=255) aaTTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCAT‑CTGTTTTAAGATGCGAAACATGAAATTCAACggagg < 2:1116977/90‑1 (MQ=255) ttCTCGAGGCGGGGCCAGAAAAAAAGACACGCACATTTTATTTTTTCTCAT‑TTGTTTTTATATGCGGAAAAAGAAAATCAACGTAGGaaa > 2:1083227/1‑90 (MQ=255) aGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCAT‑CTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAaaataaa < 2:1265197/90‑1 (MQ=255) ggCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCAT‑CTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAaaataaat < 1:141146/90‑1 (MQ=255) aaaGCGACATGCACATTTTATTTTCCCTCAT‑CTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGc < 1:1122378/90‑1 (MQ=255) aaGCGACATGCACATTTTATTTTCCCTCAT‑CTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCt > 1:193994/1‑90 (MQ=255) aaGCGACATGCACATTTTATTTTCCCTCAT‑CTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCt > 2:506464/1‑90 (MQ=255) | TGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCAT‑CTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCT > NZ_CP009273/2380261‑2380428 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |