Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	49,884	C→T	P21L (CCG→CTG)	folA →	type 3 dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	49,884	0	C	T	100.0%	157.2 / NA	48	P21L (CCG→CTG)	folA	type 3 dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/27);  total (21/27)

TCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCA  >  NZ_CP009273/49820‑49969                                                                 |                                                                                      tcAATGATCAGTCTGATTGCGGCGTTGGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGcc                                                              <  2:706305/90‑1 (MQ=255) taaaTGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGcc                                                              <  2:837070/88‑1 (MQ=255)  cAATGATCAGTCTGATTGCGGCTTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCt                                                             <  2:1099471/90‑1 (MQ=255)   aaTGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTg                                                            <  2:10527/90‑1 (MQ=255)        tCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGGCGATCTCGCCTGGTTTa                                                       >  1:1339137/1‑90 (MQ=255)         cAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGa                                                                                  >  2:995828/1‑62 (MQ=255)         cAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGa                                                                                  <  1:995828/62‑1 (MQ=255)         cAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTaa                                                      <  1:863125/90‑1 (MQ=255)         cAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTaa                                                      >  1:872599/1‑90 (MQ=255)         cAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTaa                                                      >  1:1052442/1‑90 (MQ=255)          aGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTaaa                                                     >  2:219179/1‑90 (MQ=255)          aGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTaaa                                                     >  1:1164335/1‑90 (MQ=255)          aGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTaaa                                                     >  2:893805/1‑90 (MQ=255)           gTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAAc                                                    >  1:345251/1‑90 (MQ=255)            tCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTaa                                                      >  1:183134/1‑87 (MQ=255)            tCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTaa                                                      <  2:183134/87‑1 (MQ=255)               gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCaa                                                <  2:493590/90‑1 (MQ=255)               gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCaa                                                <  2:1052442/90‑1 (MQ=255)               gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCaa                                                <  1:1293330/90‑1 (MQ=255)               gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCaa                                                >  1:368580/1‑90 (MQ=255)                aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAac                                               <  2:1122234/90‑1 (MQ=255)                    cggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCt                                           <  2:943274/90‑1 (MQ=255)                    cggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCt                                           <  2:345251/90‑1 (MQ=255)                    cggcgTTAGCGGTAGACCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCt                                           <  1:1164157/90‑1 (MQ=255)                     ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAac                                               >  1:39461/1‑85 (MQ=255)                     ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAac                                               <  2:39461/85‑1 (MQ=255)                     ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCtt                                          >  2:273677/1‑90 (MQ=255)                     ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCtt                                          >  1:1051487/1‑90 (MQ=255)                     ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCtt                                          >  2:984019/1‑90 (MQ=255)                     ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCt                                           <  1:879318/89‑1 (MQ=255)                     ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCt                                           >  2:879318/1‑89 (MQ=255)                      gcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTta                                         <  2:616922/90‑1 (MQ=255)                           aGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTtaaataa                                    <  1:1013836/90‑1 (MQ=255)                           aGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTtaaataa                                    <  2:1051487/90‑1 (MQ=255)                               gTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAAccc                                <  2:1218173/90‑1 (MQ=255)                                 aGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGt                              <  2:35192/90‑1 (MQ=255)                                 aGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGt                              <  2:872599/90‑1 (MQ=255)                                      gcgTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTa                         >  1:700144/1‑90 (MQ=255)                                         ttATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATgg                      >  1:1345550/1‑90 (MQ=255)                                         ttATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTCCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATgg                      <  1:732449/90‑1 (MQ=255)                                             cGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccg                  <  1:1206309/90‑1 (MQ=255)                                                      aaaCCCCATGCTGTGGACCCTCCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTg         <  2:1216069/90‑1 (MQ=255)                                                       aaCGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTgg        >  1:1164499/1‑90 (MQ=255)                                                         cGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGa      >  2:135490/1‑90 (MQ=255)                                                         cGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGa      <  1:273677/90‑1 (MQ=255)                                                             aTGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAac                                               >  2:577613/1‑45 (MQ=255)                                                             aTGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAac                                               <  1:577613/45‑1 (MQ=255)                                                             aTGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatca  >  1:225668/1‑90 (MQ=255)                                                                 |                                                                                      TCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCA  >  NZ_CP009273/49820‑49969

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑