Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP00927369,9000AC52.6% 53.8 / 73.8 57V370G (GTG→GGG) thiPthiamine/thiamine pyrophosphate ABC transporter permease ThiP
Reads supporting (aligned to +/- strand):  ref base A (17/10);  new base C (0/30);  total (17/40)
Fisher's exact test for biased strand distribution p-value = 6.04e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.69e-07
Rejected as consensus: Frequency below/above cutoff threshold.

GGCCGATAGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATA  >  NZ_CP009273/69811‑69981
                                                                                         |                                                                                 
ggCCGATAGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCa                                                                                   >  2:862655/1‑90 (MQ=255)
ggCCGATAGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCa                                                                                   >  1:32927/1‑90 (MQ=255)
ggCCGATAGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCa                                                                                   >  2:227986/1‑90 (MQ=255)
ggCCGATAGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCa                                                                                   >  1:580287/1‑90 (MQ=255)
   cGATAGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCt                                                                                <  2:279326/90‑1 (MQ=255)
     aTAGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGa                                                                              >  1:116609/1‑90 (MQ=255)
          tttgtggAGCAGAAAAAAGAAGCCGGAAGCCAGGCCAATCCCCGGCATGGCGAGGATCAACATGCCGCTCACCTCCAGCACCTGACCCGc                                                                         <  2:440707/89‑1 (MQ=255)
           ttgttgAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGcc                                                                        >  2:562862/1‑90 (MQ=255)
             gttgAGCAGAAAAAAGAAGCCGGTAGCCAGCAAAACCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCCCCTGACCCGCCAg                                                                      <  2:494398/90‑1 (MQ=255)
                       aaaaaGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGc                                                            >  1:665269/1‑90 (MQ=255)
                          aagaagCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCcgc                                                         >  1:682503/1‑90 (MQ=255)
                              agCCGGTAGCCAGCACAACCCCCGGCATGGGGAGGATCAACATGCCGCTCATCCCCACCCCCTGACCCGCCAGCATTTTCTGCCGCGccc                                                     <  2:989887/90‑1 (MQ=255)
                               gCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCg                                                    >  2:345029/1‑90 (MQ=255)
                                ccGGTGCCCAGCACAACCCCCGGCAGGGCGAGGAACAACACGCCCCTCATCCCCAGCCCCTGACCCGCCAGCATTTTCTGCCGCGCCCGc                                                   <  1:562862/90‑1 (MQ=255)
                                ccGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGc                                                   >  2:930592/1‑90 (MQ=255)
                                ccGGTAGCCAGCACAACCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCCCCTGACCCGCCAGCATTTTCTGCCGCGCCCGc                                                   <  2:40680/90‑1 (MQ=255)
                                ccGGTACCCACCACAACCCCCGGCAGGGGGAGGATCAACATGCCGCTCATCCCCAGCCCCTGACCCGCCAGCATTTTCTGCCGCGCCCGc                                                   <  1:760706/90‑1 (MQ=255)
                                          gCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGAc                                         >  1:884182/1‑90 (MQ=255)
                                           cacaACCCCCGGCATGGCGGGGACCAACATGCCCCCCACCCCCAGCCCCTGCCCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACt                                        <  1:279848/90‑1 (MQ=255)
                                           cacaACCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACt                                        <  2:4154/90‑1 (MQ=255)
                                           cacaACCCCCGGCATGGCGAGGATCAACATGCCGCTCATCCCCAGCCCCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACt                                        <  2:888837/90‑1 (MQ=255)
                                           cacaACCCCCGGCATGGCGAGGATCAACATGCCGCTCATCCCCAGCCCCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACt                                        <  2:109669/90‑1 (MQ=255)
                                           cacaACCCCCGGCATGGCGAGGATCAACATCCCCCTCATCCCCAGCCCCTGCCCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACt                                        <  1:749530/90‑1 (MQ=255)
                                           cacaACCCCCGGCAGGGCGAGGATCAACATCCCGCTCATCCCCAGCCCCTGCCCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACt                                        <  2:841601/90‑1 (MQ=255)
                                               aTCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGTCTGCTc                                    >  1:468123/1‑90 (MQ=255)
                                                cccccGGCAGGGCGAGGATCAACAGGCCCCTCATCCCCAGCCCCTGCCCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTcc                                   <  2:975138/89‑1 (MQ=255)
                                                    cGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATag                               >  2:232354/1‑90 (MQ=255)
                                                     ggCATGGGGAGGATCAACATGCCGCTCATCTCCAGCCCCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATagc                              <  2:797819/90‑1 (MQ=255)
                                                     ggCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATagc                              <  2:32927/90‑1 (MQ=255)
                                                     ggCAGGGCGAGGATCAACATGCCGCTCATCCCCAGCCCCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATagc                              <  2:213654/90‑1 (MQ=255)
                                                     ggCAGGGCGAGGACCAACATCCCCCTCACCCCCAGCCCCTGCCCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATagc                              <  1:104660/90‑1 (MQ=255)
                                                     ggCAGGGCGAGAATCAACATGCCGCTCATCCCCAGCCCCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATagc                              <  2:712089/90‑1 (MQ=255)
                                                       tagggCGAGAACCACCATGCCCCTCATCCCCGGCCCTTGCCCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATagcag                            <  2:715660/87‑1 (MQ=255)
                                                       cATGGGGGGGATCAACATGCCGCTCATCTCCAGCCCCTGCCCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATagcag                            <  2:645169/90‑1 (MQ=255)
                                                         tGGCGAGGATCAACATGCCGCTCATCTCCAGCCCCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACt                                        <  2:201730/76‑1 (MQ=255)
                                                         tGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACt                                        >  1:201730/1‑76 (MQ=255)
                                                            cGAGGACCACCATGCCGCTCATCCCCACCCCCTGCCCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGcatca                       <  1:59754/90‑1 (MQ=255)
                                                            cGAGAATCAACATGCCCCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGcatca                       <  2:244653/90‑1 (MQ=255)
                                                                 accAACAGGCCCCTCATCCCCAGCCCCTGCCCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTc                  <  1:544660/88‑1 (MQ=255)
                                                                 aTCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTc                  <  2:884182/90‑1 (MQ=255)
                                                                 aTCAACATGCCCCTCACCTCCAGCCCCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTc                  <  2:121869/90‑1 (MQ=255)
                                                                   cAACATGCCGCTCATCTCCAGCCCCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAg                <  2:53292/90‑1 (MQ=255)
                                                                   cAACATGCCCCTCATCTCCAGCCCCTGCCCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAg                <  2:116609/90‑1 (MQ=255)
                                                                   aaaCATCCCCCTCATCCCCACCACCTGCCCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAg                <  2:31847/89‑1 (MQ=255)
                                                                   aaaCAGGCCGCTCATCCCCAGCCCCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAg                <  2:852566/89‑1 (MQ=255)
                                                                    accgtGCCGCCCCTCCCCAGCCCCTCCCCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGc               <  1:700078/86‑1 (MQ=255)
                                                                      cttcccGCTCACCCCCACCCCCTGCCCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCAc             <  2:735698/86‑1 (MQ=255)
                                                                        tcccGCTCATCCCCAGCCCCTGCCCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTa           <  2:905217/88‑1 (MQ=255)
                                                                          ccGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACg         >  1:745811/1‑90 (MQ=255)
                                                                          ccGCTCATCCCCAGCCCCTGCCCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACg         <  2:250807/90‑1 (MQ=255)
                                                                           cccTCACCCCCAGCACCTGCCCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGc        <  2:359534/88‑1 (MQ=255)
                                                                           cGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGc        >  2:789349/1‑90 (MQ=255)
                                                                           cGCTCATCCCCAGCCCCTGCCCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGc        <  2:889946/90‑1 (MQ=255)
                                                                           cGCTCATCCCCAGCCCCGACCCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGc        <  1:224337/90‑1 (MQ=255)
                                                                            gCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGca       >  1:905217/1‑90 (MQ=255)
                                                                                aTCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGc                                                   >  1:210561/1‑42 (MQ=255)
                                                                                aTCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGc                                                   <  2:210561/42‑1 (MQ=255)
                                                                                 tctcCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATa  >  2:254057/1‑90 (MQ=255)
                                                                                       gCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGcatca                       >  1:859302/1‑63 (MQ=255)
                                                                                       gCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGcatca                       <  2:859302/63‑1 (MQ=255)
                                                                                       gCACCTGACCCGCCAGCATTTTCTGCCGAGCCCGc                                                   >  2:464214/1‑35 (MQ=38)
                                                                                         aCCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTcgcg                                           <  2:730564/41‑1 (MQ=255)
                                                                                         aCCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTcgcg                                           >  1:730564/1‑41 (MQ=255)
                                                                                         |                                                                                 
GGCCGATAGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATA  >  NZ_CP009273/69811‑69981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: