| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 645,916 | 0 | T | G | 57.1% | 1.2 / 12.0 | 14 | T11P (ACT→CCT) | citE | citrate (pro‑3S)‑lyase subunit beta |
| Reads supporting (aligned to +/- strand): ref base T (2/4); new base G (8/0); total (10/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.50e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.75e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGG > NZ_CP009273/645842‑645998 | cGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGCGGGTTTTAAGTTTTTGccg > 2:539227/1‑88 (MQ=255) aGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGGGGGGTTTTCGTTTTTTTAGCGAAACGGaaa > 1:770016/1‑90 (MQ=255) gAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGGGGGGTTTTTGTTGTTGGAGCGAAAAGGaaaaa > 1:378948/1‑88 (MQ=255) tGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGGGGGGTTTTCGTTGTTTCAGCGAAGGGGGAAACATTg > 1:404105/1‑90 (MQ=255) tGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGCGGGGTTTTCGTTGTTTGCGCGAAGCGGAAAAAATTg > 1:54767/1‑90 (MQ=255) ccATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAAtc < 1:289857/90‑1 (MQ=255) ccATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAAtc < 1:510304/90‑1 (MQ=255) ccATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAAtc < 2:770016/90‑1 (MQ=255) gCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGGGGGGTTTTCGTTGTTTCAGCGAAGGGGGAAAAAATGGCAAACCCCCCCTg > 1:595667/1‑90 (MQ=255) ttGGCACCAGGCACAAACAACATGCTGCGGCGGGGGGGGGGTTTTTGTTGTTGCAGCGAAGCGGGAAACAATGGCAATCCCCCCCTGGCa > 1:772762/1‑90 (MQ=255) acaTGCTGCGGCGGGTGGGGGGTTTTCGTTGTTTCAGCGAAGCGGAAAATATTTGTAAACCCCCCCTGGCAGGGCCCGGGAACCCCTGgc > 2:718533/1‑90 (MQ=255) gctgcGGCGGGGGGGGGGTTTTCGTTGTTGCAGCGAAGCGGAAAACATTGGCAATCCCCCCCTGGGAGAGGCGGGGGACCCCCGGcgcgg > 1:20659/1‑89 (MQ=255) ctgcGGCGGGGGGGGGTTTTTTGTTTTTGGAGCGAAGCGGAAAACATTTGCAACCCTCCCCCCGGAGAGCCGGGGTAACCCCGGcgcggg > 1:349204/1‑88 (MQ=255) gTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAggg < 2:772762/90‑1 (MQ=255) | CGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGG > NZ_CP009273/645842‑645998 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |