Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273799,3250GT63.2% 3.7 / 26.5 19V123L (GTG→TTG) ybhJhydratase
Reads supporting (aligned to +/- strand):  ref base G (0/7);  new base T (12/0);  total (12/7)
Fisher's exact test for biased strand distribution p-value = 1.98e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.45e-04
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCTCCGCATATTGCGGTCATCCATCAATATATGCGTGAGATGATGGCAGGCGGCGGCAAAATGATCCTCGGG  >  NZ_CP009273/799241‑799399
                                                                                    |                                                                          
tcactcTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCt                                                                       <  1:606916/90‑1 (MQ=255)
  actcTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGGATTTTTTTGCcccc                                                                     >  2:472115/1‑90 (MQ=255)
  actcTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGGATTTTTTTGCCTcc                                                                     >  2:27477/1‑90 (MQ=255)
   ctctGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGGATTTTTTTGGCTCCg                                                                    >  2:276825/1‑90 (MQ=255)
               ggcggcACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCTCCGCATATTGCGGTc                                                        <  1:472115/90‑1 (MQ=255)
                  ggcACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCTCCGCATATTGCGGTcatc                                                     <  2:843495/90‑1 (MQ=255)
                  ggcACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCTCCGCATATTGCGGTcatc                                                     <  2:522491/90‑1 (MQ=255)
                      cTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCTCCGCATATTGCGGTcatccatc                                                 <  2:462757/90‑1 (MQ=255)
                                tgatgaCCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTTTGCCTCCGCCTATTGCGGGCATCCATCAATATATGCg                                       >  2:323384/1‑90 (MQ=255)
                                tgatgaCCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTTTGCCTCCGCATATTTCGGGCATCCCTCAATATATGCg                                       >  2:163489/1‑90 (MQ=255)
                                tgatgaCCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTTTGCCTCCCCATATTTCGGGCATCCATCAATATATGCg                                       >  2:218438/1‑90 (MQ=255)
                                tgatgaCCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGGATTTTTTTGCCCCCGCATATTTCGGGCATCCATCAAAATATGCg                                       >  2:478303/1‑90 (MQ=255)
                                  atgaCCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTTTGCCTCCGCATATTGGGGGCATCCATTAATATATGCggg                                     >  2:611415/1‑88 (MQ=255)
                                         tGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCTCCg                                                                    <  2:202820/52‑1 (MQ=255)
                                         tGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGGATTTTTTTGCCTccc                                                                    >  1:202820/1‑51 (MQ=255)
                                         tGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGGATTTTTTTGCCTCCCCCTATTGCGGGCATCCCTCAATATATTCGGGGggtgat                              >  1:47194/1‑90 (MQ=255)
                                                   ttATCGGCGGCCCAGCGTTATGGCGGTATTTTtgt                                                                           <  2:395580/35‑1 (MQ=255)
                                                               cAGCGTTATGGCGGGATTTTTTTGCCCCCCCCTATTTCGGGCCTCCCTCAATATATGCGGGGGGTGATGGCCGGCGGCGGGCAAAAGATc        >  1:367165/1‑90 (MQ=255)
                                                                 gCGTTATGGCGGTATTTTTGTGCCTCCGCATATTGCGGTCATCCATCAATATATGCGTGAGATGATGGCAGGCGGCGGCAAAATGATCCt      <  2:108784/90‑1 (MQ=255)
                                                                     tATGGCGGTATTTTTTTGCCTCCCCATATTGCGGTCATCCCTCAATATATGCGTGAGATGATGGCAGGGGGGGGGAAAAAGATCCTCggg  >  1:194736/1‑90 (MQ=255)
                                                                                    |                                                                          
TCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCTCCGCATATTGCGGTCATCCATCAATATATGCGTGAGATGATGGCAGGCGGCGGCAAAATGATCCTCGGG  >  NZ_CP009273/799241‑799399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: