| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,725,059 | 0 | T | G | 61.1% | 8.2 / 19.2 | 18 | G572G (GGT→GGG) | lhr | ATP‑dependent helicase |
| Reads supporting (aligned to +/- strand): ref base T (0/7); new base G (11/0); total (11/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.14e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.92e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACCATTCCGGATCGTGGCATGTATAGCGTGTTATTACCCGAAGGTGAAGAAAAGGCCGGTTCGCGGCGGGTGGGTGAACTGGATGAGGAGATGGTATATGAGTCGCGGGTGAACGACATTATCACTCTCGGCGCTACCTCATGGCGGATCCAGCAAATCACC > NZ_CP009273/1724985‑1725146 | aCCATTCCGGATCGTGGCATGTATAGCGTGTTATTACCCGAAGGTGAAGAAAAGGCCGGTTCGCGGCGGGTGGGTGAACTGGATgaggag < 1:102824/90‑1 (MQ=255)aCCATTCCGGATCGTGGCATGTATAGCGTGTTATTACCCGAAGGTGAAGAAAAGGCCGGTTCGCGGCGGGTGGGTGAACTGGATgaggag < 2:386026/90‑1 (MQ=255) aTTCCGGATCGTGGCATGTATAGCGTGTTATTACCCGAAGGTGAAGAAAAGGCCGGTTCGCGGCGGGTGGGTGAACTGGATGAGGAGATg < 2:269984/90‑1 (MQ=255) gTGGCATGTATAGCGTGTTATTACCCGAAGGTGAAGAAAAGGCCGGTTCGCGGCGGGGGGGGGGAATGGGTGGGGAGAGGGGATATGAgg > 1:633615/1‑89 (MQ=255) gTGGCATGTATAGCGTGTTATTACCCGAAGGTGAAGAAAAGGCCGGTTCGCGGCGGGGGGGGGAACTGGGTGAGGAGATGGGATATGAGt > 2:225725/1‑90 (MQ=255) gTGGCATGTATAGCGTGTTATTACCCGAAGGTGAAGAAAAGGCCGGTTCGCGGCGGGGGGGGGAAATGGGTGAGGAGATGGGATATTAgg > 2:865414/1‑89 (MQ=255) gTGGCATGTATAGCGTGTTATTACCCGAAGGTGAAGAAAAGGCCGGGTCGCGGCGGGGGGGGGGACCGGTTGGGGGGGAGGTATATGAgc > 1:923392/1‑89 (MQ=255) aGCGTGTTATTACCCGAAGGTGAAGAAAAGGCCGGTTCGCGGCGGGTGGGTGAACTGGATGAGGAGATGGTATATGAGTCGCGGGTGAAc < 1:410055/90‑1 (MQ=255) gCGTGTTATTACCCGAAGGTGAAGAAAAGGCCGGTTCGCGGCGGGGGGGGGAACTGGGTGAGGGGATGGGATATGAGTCGCGGGGGGACg > 1:627736/1‑90 (MQ=255) gCGTGTTATTACCCGAAGGTGAAGAAAAGGCCGGTTCGCGGCGGGGGGGGGAACTGGATGAGGAGATGGTATATGAGTCGCGGGGGGACg > 2:894957/1‑90 (MQ=255) gtgtTATTACCCGAAGGTGAAGAAAAGGCCGGTTCGCGGCGGGTGGGTGAACTGGATGAGGAGATGGTATATGAGTCGCGGGTGAACGAc < 2:470315/90‑1 (MQ=255) gtTATTACCCGAAGGTGAAGAAAAGGCCGGTTCGCGGCGGGGGGGGGAACTGGATGAGGAGATGGGATATGAGGCGCGGGGGGACGACAt > 2:457315/1‑90 (MQ=255) ttattaCCCGAAGGTGAAGAAAAGGCCGGTTCGCGGCGGGGGGGGGAACTGGATGAGGAGATGGGATATGAGTCGCGGGTGGACGACAtt > 2:17525/1‑90 (MQ=255) gAAGGTGAAGAAAAGGCCGGTTCGCGGCGGGTGGGTGAACTGGATGAGGAGATGGTATATGAGTCGCGGGTGAACGACATTATCACtctc < 1:457315/90‑1 (MQ=255) gAAGGTGAAGAAAAGGCCGGTTCGCGGCGGGGGGGGGAACTGGATGGGGGGGTGGGATATGAGTCGCGGGGGGAGGACATTATCACtctc > 1:659099/1‑90 (MQ=255) tGAAGAAAAGGCCGGTTCGCGGCGGGTGGGTGAACTGGATGAGGAGATGGTATATGAGTCGCGGGTGAACGACATTATCACTCTCGgcgc < 2:240875/90‑1 (MQ=255) ggTTCGCGGCGGGGGGGGGAACTGGATGAGGAGATGGTATATGAGGCGCGGGGGGAAGACATTATTACTCTCGGCGCTACCTCCTGGCgg > 1:379297/1‑90 (MQ=255) ggTTCGCGGCGGGGGGGGGAACTGGATGAGGAGATGGGATATGAGTCGCGGGGGGACGGCATTTTCACTCTCGGGGCTACCCCATGGCgg > 1:719658/1‑90 (MQ=255) ggtgAACTGGATGAGGAGATGGTATATGAGTCGCGGGTGAACGACATTATCACTCTCGGCGCTACCTCATGGCGGATCCAGCAAATCAcc < 1:166664/90‑1 (MQ=255) | ACCATTCCGGATCGTGGCATGTATAGCGTGTTATTACCCGAAGGTGAAGAAAAGGCCGGTTCGCGGCGGGTGGGTGAACTGGATGAGGAGATGGTATATGAGTCGCGGGTGAACGACATTATCACTCTCGGCGCTACCTCATGGCGGATCCAGCAAATCACC > NZ_CP009273/1724985‑1725146 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |