| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 169,398 | 0 | A | G | 52.6% | 13.8 / 27.3 | 19 | M484V (ATG→GTG) | fhuB | Fe(3+)‑hydroxamate ABC transporter permease FhuB |
| Reads supporting (aligned to +/- strand): ref base A (0/9); new base G (10/0); total (10/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.08e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.65e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGCGGTGACGCTGTTGATCATTATGATCGCCGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGATGGCGTTAAGCACCGCGTTCACCATGCTTTTGATGATGTTGCAGGCAAGTGGTGACCCGCGAATGGCGCAAGTGCTGACCTG > NZ_CP009273/169322‑169480 | ggcggTGACGCTGTTGATCATTATGATCGCCGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGATGGCGTTAAGCAc < 2:771799/90‑1 (MQ=255)ggcggTGACGCTGTTGATCATTATGATCGCCGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGATGGCGTTAAGCAc < 2:711850/90‑1 (MQ=255) ttATGATCGCCGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGGGGGGGGTAAGCACCCCGGTCACCCTCCtttttt > 2:571809/1‑88 (MQ=255) aTGATCGCCGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGGTGGCGTTAAGCACCGCGt > 1:22904/1‑73 (MQ=255) aTGATCGCCGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGATGGCGTTAAGCACCGCGt < 2:22904/73‑1 (MQ=255) tGATCGCCGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGATGGCGTTAAGCACCGCGTTCACCATGCTTTtgatga < 1:188314/90‑1 (MQ=255) aTCGCCGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGGGGGGGGTAAGGACCCCGTTCCCCCTGCTTTTTttgatg > 2:188314/1‑90 (MQ=255) ccgccgGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGATGGCGTTAAGCACCGCGTTCACCATGCTTTTGATGATGTTGc < 1:164736/90‑1 (MQ=255) ggccgCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGATGGCGTTAAGCACCGCGTTCACCATGCTTTTGATGATGTTGCAGGCa < 2:254055/90‑1 (MQ=255) tCCCCACACCGTATGTTACTGGCGGGGATGGCGTTAAGCACCGCGTTCACCATGCTTTTGATGATGTTGCAGGCAAGTGGTGACCCGCGa < 1:400127/90‑1 (MQ=255) acacCGTATGTTACTGGCGGGGGGGGGGGTAAGCACCCCGCTCCCCACGCTTTTTTTGATGTTGGAGGCAAGGGGGGAGCCCCGGAAGgc > 2:860894/1‑90 (MQ=255) ccGTATGTTACTGGCGGGGATGGCGTTAAGCACCGCGTTCACCATGCTTTTGATGATGTTGCAGGCAAGTGGTGACCCGCGAATGGCGCa < 1:470384/90‑1 (MQ=255) ccGTATGTTACTGGCGGGGATGGCGTTAAGCACCGCGTTCACCATGCTTTTGATGATGTTGCAGGCAAGTGGTGACCCGCGAATGGCGCa < 1:678914/90‑1 (MQ=255) gTATGTTACTGGCGGGGGTGGCGTTAAGCACCGCGTTCACCATGCTTTTTTTGATGTTTCAGGCAAGTGGGGACCCGCGAATGGCGCAAg > 1:706747/1‑90 (MQ=255) gTATGTTACTGGCGGGGGGGGCGGTTAGAACCGCGCTCACCCCGCTTTTTTTGATGTTTGAGGCAAGGGGGGACCCCCGAAAGgcgggag > 1:381987/1‑86 (MQ=255) tACTGGCGGGGGTGGGGTTTAAAACCCCGGTCACCCTGCTTTTTTTGATGTTGGAGGCAAGGGGGGGCCCGCGGATGGCGCAAGTGCTGa > 2:873403/1‑90 (MQ=255) tACTGGCGGGGGTGGCGGTTAGCACCGCGGTCACCATGCTTTTTTTGATGTTGGAGGCAAGTGGGGGCCCCCGAATGGGGCAAGTGGTGa > 1:459698/1‑90 (MQ=255) cTGGCGGGGGGGGGGTTAAGCACCGCGTTCACCATGCTTTTTTTGATGTTGGAGGGAAGGGGGGGCCCCCGGAAGGGGCCAGTGCTGAcc > 2:121845/1‑90 (MQ=255) ggCGGGGGGGGCGGTAAGCACCGCGGTCACCATGCTTTTTTTGGTGTTGGGGGGGAGGGGGGGGCCGCGAAAGGCGCCAAGGCTGAccgt > 2:512703/1‑88 (MQ=255) | GGCGGTGACGCTGTTGATCATTATGATCGCCGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGATGGCGTTAAGCACCGCGTTCACCATGCTTTTGATGATGTTGCAGGCAAGTGGTGACCCGCGAATGGCGCAAGTGCTGACCTG > NZ_CP009273/169322‑169480 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |