| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 172,920 | 0 | C | A | 61.1% | 1.1 / 20.6 | 18 | P443T (CCG→ACG) | clcA | H(+)/Cl(‑) exchange transporter ClcA |
| Reads supporting (aligned to +/- strand): ref base C (1/6); new base A (11/0); total (12/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.77e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.86e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGACCGATAACTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAACCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATCAGCCAGCGAGAAT > NZ_CP009273/172837‑173009 | tGACCGATAACTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGGAAACGCtat > 1:845931/1‑90 (MQ=255)tGACCGATAACTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAAACGCcat > 1:548849/1‑90 (MQ=255) ccAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAAACGCTATACTCGGCGATTCt > 2:117668/1‑90 (MQ=255) gCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAAACCCTATACTCGGCGATtttttc > 2:591741/1‑88 (MQ=255) ggATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAACCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCaa < 1:329394/89‑1 (MQ=255) attaCCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGGAAACGCTAAACACGGCGGTTCTTTGGCGCCCGCTGGGCAAACaa > 2:548464/1‑89 (MQ=255) ggTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAACCGCTATACTCGGCGATTCTTGCGCGCACGCCGGCAAAACAAGAAGCt > 2:178713/1‑90 (MQ=255) ggTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAAACGCTATAATCGGGGATTCTTTCGCGCACGCTGGCAAAAAAGGaaacc > 2:329394/1‑87 (MQ=255) gcgcAACACTATTAGCGCAATTTACCGGCGGGAAACCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAAc < 2:548849/90‑1 (MQ=255) aaCACTATTAGCGCAATTTACCGGCGGGGAAACGCTATACTCGGCGGTTCTTTCGCGCCCGCTGGCAAAACAGGAAACTGAGCAAATGgc > 2:234288/1‑90 (MQ=255) cacTATTAGCGCAATTTACCGGCGGGAAACCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGgcgc < 1:412347/90‑1 (MQ=255) cacTATTAGCGCAATTTACCGGCGGGAAACCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTAgcgc < 1:591741/90‑1 (MQ=255) cgcAATTTACCGGCGGGAAACCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCaa < 2:595178/57‑1 (MQ=255) cgcAATTTACCGGCGGGAAAACGCTATACTCGGCGATTCTTGCGCGCACGCTGGCaa > 1:595178/1‑57 (MQ=255) aaTTTACCGGCGGGAAAACGCCATACTCCGCGGTTCTTGCGCGCACGCCGGCAAAAAAGGAAGCTGAGCAACTGGCGCGAAGCAAAGCCg > 2:174714/1‑90 (MQ=255) ttACCGGCGGGAAACCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCAt < 1:863390/90‑1 (MQ=255) ccGGCGGGAAAACGCCATACTCCGCGGTTCTTGCGCGCCCCCTGGCAAAACAGGAAGGTGAGCAACTGGCGCGGAGCAAGGCCGCATcag > 2:321304/1‑90 (MQ=255) gggAAAACGCTATACTCGGCGATTTTTTCGCGCCCGCTGGCAAAACAAGAAGCTGAGCAACTGGCGCGAAGCAAAGCCGCATcagcccgc > 2:863390/1‑90 (MQ=255) ccGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATCAGCCAGCGAGAAt < 1:174714/90‑1 (MQ=255) ccGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATCAGCCAGCGAGAAt < 2:330155/90‑1 (MQ=255) ccGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATCAGCCAGCGAGAAt < 1:548464/90‑1 (MQ=255) ccGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATCAGCCAGCGAGAAt < 2:653787/90‑1 (MQ=255) | TGACCGATAACTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGCGCAACACTATTAGCGCAATTTACCGGCGGGAAACCGCTATACTCGGCGATTCTTGCGCGCACGCTGGCAAAACAGGAAGCTGAGCAACTGGCGCGAAGCAAGGCCGCATCAGCCAGCGAGAAT > NZ_CP009273/172837‑173009 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |