| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 822,955 | 0 | T | G | 57.1% | 16.2 / 27.0 | 21 | T495P (ACC→CCC) | ybhF | ATP‑binding cassette domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base T (1/8); new base G (12/0); total (13/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.42e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.41e-05 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGC > NZ_CP009273/822886‑823037 | gggtggTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGAACGCCGGGAg > 2:48585/1‑90 (MQ=255) aTCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTtcgtcg < 2:621897/90‑1 (MQ=255) gTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCa < 2:572888/63‑1 (MQ=255) gTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGGCa > 1:572888/1‑63 (MQ=255) gTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAAAGCCCGAAGTCGGGTCGTCGAGaaaa > 1:464199/1‑89 (MQ=255) ccATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGAAAAGCCGGAAGGCGGGGCGTCGAGCAACAGAAAGTGCCGGTCAt > 1:481615/1‑90 (MQ=255) aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGAAGGTGGTTTGTCGGGAAAAAGAAAGTCCGGTTCTTGc > 1:319230/1‑90 (MQ=255) aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACCCCGGAAGGCGGGTCGTCGGGAAAAAAAAAGTCCGGGTTATGc > 2:52180/1‑90 (MQ=255) cTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATc < 2:293094/90‑1 (MQ=255) gTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGCAACGCCGGGAGACGGGTCGGCGAGAAAAAAAAAGTCCCGGTCATGCacccg > 2:31441/1‑86 (MQ=255) tGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGCAACGCCGGAAGTCGGGTCGTCGAGAAACAGAATGTCCGGGTCATGCATCAGCg > 1:139366/1‑90 (MQ=255) tgtgCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAAc < 2:510733/90‑1 (MQ=255) tgtgCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAAc < 1:48585/90‑1 (MQ=255) tgtgCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAAc < 2:403993/90‑1 (MQ=255) gtgCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGAAGGCGGGTCGTCGAGAAACAGAAAGTCCGGGTCATGCATCAGCGaaaa > 1:266994/1‑88 (MQ=255) gCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGAAGTCGGGTCGTCGAGAAACAAAATGTCCGGTTCATGCATCAGCGAACAgg > 1:293094/1‑90 (MQ=255) gCAGCCAAAATTCACGGCGGGGGAGGGGGGCAACGCCGGGAGTCGGTTCGTCGAGAAAAAGAAAGTCCGGGTCATGCATCAGCGAACAgg > 1:827814/1‑90 (MQ=255) gCAGCCAAAATTCACGGCGGGGGAGGGGGGCAACGCCGGAAGTCGGTTCGTCGAGAAAAAGAAAGTCCGGGTCATGCATCAGCGAACAgg > 1:90283/1‑90 (MQ=255) aGCCAAAATTCACGGCGGGGGGGGGGGGCAAAGCCGGGAGGCGGGTCGTCGAGAAAAAGAAAGTCCGGGTCATGCATCAGCGGACAGgcc > 1:510733/1‑90 (MQ=255) cggcggGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGc < 2:139366/90‑1 (MQ=255) cggcggGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGc < 2:827814/90‑1 (MQ=255) | GGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGC > NZ_CP009273/822886‑823037 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |