| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,089,571 | 0 | A | G | 55.6% | 8.6 / 19.0 | 18 | I83V (ATT→GTT) | hisF | imidazole glycerol phosphate synthase subunit HisF |
| Reads supporting (aligned to +/- strand): ref base A (1/7); new base G (10/0); total (11/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.51e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.38e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAG‑ATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACC > NZ_CP009273/2089486‑2089652 | ttCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGATTaa > 1:461280/1‑90 (MQ=255) aTGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTTAGTCTCTgg > 1:714222/1‑90 (MQ=255) tGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGTCTCTGGa > 1:736990/1‑90 (MQ=255) gggTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGGTTAAGtc > 1:486006/1‑61 (MQ=255) gggTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGtc < 2:486006/61‑1 (MQ=255) tGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAG‑ATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCa < 2:173468/90‑1 (MQ=255) tGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAG‑ATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCa < 2:461280/90‑1 (MQ=255) gATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAAGCTTTGGGGGAAGGCCCGGAAAATTTTTTCTTTTGG‑GCGGGTAAAAATTTCAt > 2:699095/1‑90 (MQ=255) aTCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGTCTCTGGAAG‑ATGCCGCGAAAAATCTTTTCTTTTGCGCGGGTAAAAATTCCATc > 1:542879/1‑90 (MQ=255) gACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAG‑ATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAAc < 2:624035/90‑1 (MQ=255) aCATTCCGTTTTGTGTGGCGGGGGGGGTTAAGTCTCTGGAAG‑ATGCCGCGAAAATTCTTTCCTTTTGCGCGGATAAAATTTCCATCAAct > 1:347657/1‑90 (MQ=255) ttCCGTTTTGTGTGGCGGGGGGGGTTAAATCTCTGGAAG‑ATGCCGCGAAAATTTTTTTCTTTTGGGGGGGTAAAAATTTCATCAACtctc > 2:594313/1‑90 (MQ=255) ttCCGTTTTGTGTGGCGGGGGGGGTTAAATCTCTGGAAG‑ATGCCGCGAAAATTTTTTTCTTTTGGGCGGGTAAAATTTTCATCAACtctc > 2:709882/1‑90 (MQ=255) tCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAG‑ATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTcc < 1:594313/90‑1 (MQ=255) gTTTTGTGTGGCGGGGGGGGTTAAGTCTCTGGAAA‑AAGCCCCGAAAATTTTTTTCTTTTGGGGGGGTAAAAATTTCATCAACCCTTCtct > 1:658280/1‑88 (MQ=255) tttGTGTGGCGGGTGGGATTAAGTCTCTGGAAG‑ATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTgcgc < 1:828404/90‑1 (MQ=255) gCGGGGGGGGTTAAGTCTCTGGGAG‑ATGCCGCGAAAATTTTTTTCTTTGGGGCGGGTAAAAATTTCATCAACTCTCCTGCGCTGGGAGAc > 2:8090/1‑90 (MQ=255) cGGGTGGGATTAAGTCTCTGGAAG‑ATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGAcc < 2:736990/90‑1 (MQ=255) | TTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAG‑ATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACC > NZ_CP009273/2089486‑2089652 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |